Difference of regulatory T cells, IL10, IL6, IFNγ, and IDO levels in female with high ASA and virgin: A research article
| Autor: | Indra Gusti Mansur, Mohamad Sadikin, Ichramsjah A. Rahman, Nani Sari Murni |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male Regulatory T cell Immunology Population chemical and pharmacologic phenomena T-Lymphocytes Regulatory 03 medical and health sciences Interferon-gamma Young Adult 0302 clinical medicine Immune system Pregnancy Agglutination Tests medicine Immune Tolerance Immunology and Allergy Humans Indoleamine-Pyrrole 2 3 -Dioxygenase education Sexual Abstinence education.field_of_study 030219 obstetrics & reproductive medicine biology business.industry Interleukin-6 Obstetrics and Gynecology FOXP3 hemic and immune systems medicine.disease Sperm Spermatozoa Interleukin-10 Interleukin 10 030104 developmental biology medicine.anatomical_structure Fertility Reproductive Medicine biology.protein Female Antibody business Infertility Female Signal Transduction |
| Zdroj: | Journal of reproductive immunology. 141 |
| ISSN: | 1872-7603 |
| Popis: | The maternal immune system requires tolerance for conception to occur. It is not only the balance of Th 1/Th2 that plays a role in pregnancy, but also the regulatory T cells (Tregs) that regulate the important role in pregnancy. One cause of failure in pregnancy is due to immunological factors, including antisperm antibodies (ASA). About 10-30% of infertile couples are caused by ASA. Th1 secretes Interferon γ (IFNγ). IFNγ is also an inducer indoleamin 2,3 dioksigenase (IDO). Cooperation between Tregs and IDO will induce tolerance for pregnancy.Th2 secretes most ofinterleukin (IL)10. Increased IL10 and decreased IL6 occur during pregnancy. The aim of this study was to analyse difference of Tregs, IL10, IL6, IFNγ, and IDO levels in female with high ASA and virgin.Samples with high ASA were examined ASA titres using the husband's sperm auto-agglutination test (HSAaT) method.49 samples were analysed. Tregs were evaluated using flowcytometry with the human forkhead box P3 (FoxP3) staining kit of Biotech and Device.Level of IL10, IL6, and IFNγ was determined using an Abcam ELISA kit. Level of IDO was determined using an RnD ELISA kit. The data were analysed using the Mann-whitney tests.There are differences in the Tregs population (p = 0.0000.05) but there is no difference IL10, IL6, IFNγ, and IDO levels in female with high ASA and virgin (p 0.1400.05, p 0.6800.05, p 0.2040.05, and p 0.3620.05).High ASA affects of the Tregs population but has no effect on cytokines IL10, IL6, IFNγ, and IDO. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect