Simultaneous quantification of losartan and active metabolite in human plasma by liquid chromatography–tandem mass spectrometry using irbesartan as internal standard
| Autor: | Budi Prasaja, Lucy Sasongko, Windy Lusthom, Matthew Grigg, Yahdiana Harahap, Hardiyanti |
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| Rok vydání: | 2009 |
| Předmět: |
Quality Control
Clinical Biochemistry Tetrazoles Pharmaceutical Science Tandem mass spectrometry Mass spectrometry High-performance liquid chromatography Losartan Analytical Chemistry Irbesartan Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Drug Discovery medicine Humans Chromatography High Pressure Liquid Spectroscopy Chromatography Chemistry Biphenyl Compounds Selected reaction monitoring Reproducibility of Results Reference Standards Angiotensin II Calibration Indicators and Reagents Angiotensin II Type 1 Receptor Blockers medicine.drug |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 49:862-867 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2009.01.007 |
| Popis: | A simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method employing electronspray ionization was developed and validated for quantification of losartan and its carboxylic acid metabolite in human plasma using irbesartan as internal standard (IS). Following a simple pretreatment procedure, the analytes were separated using a gradient mobile phase on reverse phase C18 column. Selected reaction monitoring was specific for losartan, losartan acid and irbesartan. The method validation demonstrated the specificity, lower limit of quantification, accuracy and precision of measurements. The assay exhibited a linear dynamic range of 2.0-400 ng/mL for losartan and 1.85-370 ng/mL for losartan acid. A run time of 3.5 min for each sample made it possible to analyze more than 200 samples per day. The validated method has been successfully used to analyze human plasma samples for application in bioavailability/bioequivalence studies. |
| Databáze: | OpenAIRE |
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