Distinct Physiologic Properties of Microglia and Blood-Borne Cells in Rat Brain Slices After Permanent Middle Cerebral Artery Occlusion
| Autor: | Oliver Kann, Konstantin Prass, Susan A. Lyons, Felix Knapp, Helmut Kettenmann, Carsten Ohlemeyer, Ulrich Dirnagl, Frank Wiegand, Andrea Pastor |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Pathology medicine.medical_specialty Patch-Clamp Techniques Potassium Channels Population Ischemia Brain Ischemia Immunophenotyping Membrane Potentials 030218 nuclear medicine & medical imaging 03 medical and health sciences Organ Culture Techniques 0302 clinical medicine Cell Movement medicine.artery Leukocytes medicine Animals Patch clamp Rats Wistar education Cerebral Cortex education.field_of_study Microscopy Video Microglia Inward-rectifier potassium ion channel business.industry Infarction Middle Cerebral Artery medicine.disease Rats medicine.anatomical_structure Neurology Middle cerebral artery Potassium Neurology (clinical) Cardiology and Cardiovascular Medicine business Infiltration (medical) 030217 neurology & neurosurgery |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1559-7016 0271-678X |
| Popis: | The authors investigated the time course of leukocyte infiltration compared with microglial activation in adult rat brain slices after permanent middle cerebral artery occlusion (MCAO). To distinguish peripheral leukocytes from microglia, the blood cells were prelabeled in vivo with Rhodamine 6G (Rhod6G) IV before induction of ischemia. At specific times after infarct, invading leukocytes, microglia, and endothelial cells were labeled in situ with isolectin (IL)B4-FITC (ILB4). Six hours after MCAO only a few of the ILB4+ cells were colabeled by Rhod6G. These cells expressed the voltage-gated inwardly and outwardly rectifying K+ currents characteristic of macrophages. The majority of the ILB4+ cells were Rhod6G− and expressed a lack of voltage-gated channels, recently described for ramified microglial cells in brain slices, or exhibited only an inward rectifier current, a unique marker for cultured (but unstimulated) microglia. Forty-eight hours after MCAO, all blood-borne and the majority of Rhod6G− cells expressed outward and inward currents indicating that the intrinsic microglial population exhibited physiologic features of stimulated, cultured microglia. The ILB4+/Rhod6G− intrinsic microglial population was more abundant in the border zone of the infarct and their morphology changed from radial to ameboid. Within this zone, the authors observed rapidly migrating cells and recorded this movement by time-lapse microscopy. The current findings indicate that microglial cells acquire physiologic features of leukocytes at a later time point after MCAO. |
| Databáze: | OpenAIRE |
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