Tinkering with the C-function : a molecular frame for the selection of double flowers in cultivated roses
| Autor: | Sylvie Baudino, Véronique Boltz, Philippe Vergne, Nicolas B. Langlade, Olivier Raymond, Mohammed Bendahmane, Marion Maene, Annick Dubois |
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| Přispěvatelé: | Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales (LBVPAM), Université Jean Monnet [Saint-Étienne] (UJM), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales (BVPAM), Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
0106 biological sciences
Models Anatomic Gynoecium Stamen lcsh:Medicine Plant Biology MADS Domain Proteins Flowers Rosa 01 natural sciences 03 medical and health sciences Plant Biology/Plant Genetics and Gene Expression Plant Biology/Plant Growth and Development Gene Expression Regulation Plant Botany Arabidopsis thaliana [SDV.BV]Life Sciences [q-bio]/Vegetal Biology lcsh:Science Domestication Molecular Biology In Situ Hybridization ComputingMilieux_MISCELLANEOUS 030304 developmental biology Hybrid Plant Proteins 0303 health sciences Volatile Organic Compounds Vegetal Biology Multidisciplinary biology Agamous Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling lcsh:R fungi food and beverages Gene Expression Regulation Developmental Genetic Variation biology.organism_classification Phenotype Developmental Biology/Plant Growth and Development RHAG biology.protein Hybridization Genetic lcsh:Q Petal Biologie végétale 010606 plant biology & botany Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2010, 5 (2) (9288), pp.10-1371 PLoS ONE, 2010, 5 (2) (9288), pp.10-1371. ⟨10.1371/journal.pone.0009288⟩ PLoS ONE, Public Library of Science, 2010, 5 (2) (9288), pp.10-1371. ⟨10.1371/journal.pone.0009288⟩ PLoS ONE, Vol 5, Iss 2, p e9288 (2010) Plos One 2 (5), 12 p.. (2010) |
| ISSN: | 1932-6203 |
| Popis: | Background Roses have been cultivated for centuries and a number of varieties have been selected based on flower traits such as petal form, color, and number. Wild-type roses have five petals (simple flowers), whereas high numbers of petals (double flowers) are typical attributes of most of the cultivated roses. Here, we investigated the molecular mechanisms that could have been selected to control petal number in roses. Methodology/Principal Findings We have analyzed the expression of several candidate genes known to be involved in floral organ identity determination in roses from similar genetic backgrounds but exhibiting contrasting petal numbers per flower. We show that the rose ortholog of AGAMOUS (RhAG) is differentially expressed in double flowers as compared to simple flowers. In situ hybridization experiments confirm the differential expression of RhAG and demonstrate that in the double-flower roses, the expression domain of RhAG is restricted toward the center of the flower. Conversely, in simple-flower roses, RhAG expression domain is wider. We further show that the border of RhAG expression domain is labile, which allows the selection of rose flowers with increased petal number. Double-flower roses were selected independently in the two major regions for domestication, China and the peri-Mediterranean areas. Comparison of RhAG expression in the wild-type ancestors of cultivated roses and their descendants both in the European and Chinese lineages corroborates the correlation between the degree of restriction of RhAG expression domain and the number of petals. Our data suggests that a restriction of RhAG expression domain is the basis for selection of double flowers in both the Chinese and peri-Mediterranean centers of domestication. Conclusions/Significance We demonstrate that a shift in RhAG expression domain boundary occurred in rose hybrids, causing double-flower phenotype. This molecular event was selected independently during rose domestication in Europe/Middle East and in China. |
| Databáze: | OpenAIRE |
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