Trans-Membrane Area Asymmetry Controls the Shape of Cellular Organelles
| Autor: | Beznoussenko, Galina V, Pilyugin, Sergei S, Geerts, Willie J C, Kozlov, Michael M, Burger, Koert N J, Luini, Alberto, Derganc, Jure, Mironov, Alexander A, Sub Cryo - EM, Cryo-EM |
|---|---|
| Přispěvatelé: | Sub Cryo - EM, Cryo-EM |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Endosome
lipidna membrana Biology Membrane Fusion Models Biological complex mixtures Golgijev aparat Catalysis Article Inorganic Chemistry lcsh:Chemistry symbols.namesake kiss-and-run model Organelle udc:577 Animals Humans Organelle Shape Physical and Theoretical Chemistry Rats Wistar trans-membrane area asymmetry asimetrija Molecular Biology lcsh:QH301-705.5 Spectroscopy Vesicle Organic Chemistry General Medicine COPI Intracellular Membranes Golgi apparatus intra-Golgi transport digestive system diseases Computer Science Applications Cell biology Rats Protein Transport lcsh:Biology (General) lcsh:QD1-999 Membrane curvature Golgi cisterna symbols organelle shape lipid membrane asymmetry HeLa Cells |
| Zdroj: | International journal of molecular sciences (Online) 16 (2015): 5299–5333. doi:10.3390/ijms16035299 info:cnr-pdr/source/autori:Beznoussenko, Galina V.; Pilyugin, Sergei S.; Geerts, Willie J. C.; Kozlov, Michael M.; Burger, Koert N. J.; Luini, Alberto; Derganc, Jure; Mironov, Alexander A./titolo:Trans-Membrane Area Asymmetry Controls the Shape of Cellular Organelles/doi:10.3390%2Fijms16035299/rivista:International journal of molecular sciences (Online)/anno:2015/pagina_da:5299/pagina_a:5333/intervallo_pagine:5299–5333/volume:16 International Journal of Molecular Sciences Volume 16 Issue 3 Pages 5299-5333 International Journal of Molecular Sciences, Vol 16, Iss 3, Pp 5299-5333 (2015) International Journal of Molecular Sciences, 16(3), 5299. MDPI AG International journal of molecular sciences, vol. 16, no. 3, pp. 5299-5333, 2015. |
| ISSN: | 1661-6596 1422-0067 |
| DOI: | 10.3390/ijms16035299 |
| Popis: | Membrane organelles often have complicated shapes and differ in their volume, surface area and membrane curvature. The ratio between the surface area of the cytosolic and luminal leaflets (trans-membrane area asymmetry (TAA)) determines the membrane curvature within different sites of the organelle. Thus, the shape of the organelle could be critically dependent on TAA. Here, using mathematical modeling and stereological measurements of TAA during fast transformation of organelle shapes, we present evidence that suggests that when organelle volume and surface area are constant, TAA can regulate transformation of the shape of the Golgi apparatus, endosomal multivesicular bodies, and microvilli of brush borders of kidney epithelial cells. Extraction of membrane curvature by small spheres, such as COPI-dependent vesicles within the Golgi (extraction of positive curvature), or by intraluminal vesicles within endosomes (extraction of negative curvature) controls the shape of these organelles. For instance, Golgi tubulation is critically dependent on the fusion of COPI vesicles with Golgi cisternae, and vice versa, for the extraction of membrane curvature into 50–60 nm vesicles, to induce transformation of Golgi tubules into cisternae. Also, formation of intraluminal ultra-small vesicles after fusion of endosomes allows equilibration of their TAA, volume and surface area. Finally, when microvilli of the brush border are broken into vesicles and microvilli fragments, TAA of these membranes remains the same as TAA of the microvilli. Thus, TAA has a significant role in transformation of organelle shape when other factors remain constant. |
| Databáze: | OpenAIRE |
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