Nonisothermal bioreactors in the treatment of vegetation waters from olive oil: laccase versus syringic acid as bioremediation model
| Autor: | A. Attanasio, Damiano Gustavo Mita, P. Canciglia, Stefano Sicuranza, Silvana Di Martino, Umberto Bencivenga, V. Grano, Sergio Rossi, L. Fraconte, Nadia Diano |
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| Přispěvatelé: | A., Attanasio, Diano, Nadia, V., Grano, S., Sicuranza, S., Rossi, U., Bencivenga, L., Fraconte, S., DI MARTINO, P., Canciglia, D. G., Mita |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Industrial Waste
DECOLORIZATION MEMBRANES Catalysis Water Purification chemistry.chemical_compound Bioreactors Phenols Gallic Acid Bioreactor Plant Oils Computer Simulation POLLUTANTS Olive Oil DETOXIFICATION Trametes versicolor Laccase Chromatography OXIDATIVE ENZYMES biology PLEUROTUS OSTREATUS Basidiomycota MILL WASTE WATER MATTER TRANSPORT IMMOBILIZATION ADVANTAGES technology industry and agriculture Temperature Substrate (chemistry) Agriculture Membranes Artificial Syringic acid Hydrogen-Ion Concentration biology.organism_classification Enzymes Immobilized Enzyme Activation Membrane Biodegradation Environmental chemistry Models Chemical Biotechnology Nuclear chemistry |
| Popis: | Laccase from Trametes versicolor was immobilized by diazotization on a nylon membrane grafted with glycidil methacrylate, using phenylenediamine as spacer and coupling agent. The behavior of these enzyme derivatives was studied under isothermal and nonisothermal conditions by using syringic acid as substrate, in view of the employment of these membranes in processes of detoxification of vegetation waters from olive oil mills. The pH and temperature dependence of catalytic activity under isothermal conditions has shown that these membranes can be usefully employed under extreme pH and temperatures. When employed under nonisothermal conditions, the membranes exhibited an increase of catalytic activity linearly proportional to the applied transmembrane temperature difference. Percentage activity increases ranging from 62% to 18% were found in the range of syringic acid concentration from 0.02 to 0.8 mM, when a difference of 1 degrees C was applied across the catalytic membrane. Because the percentage activity increase is strictly related to the reduction of the production times, the technology of nonisothermal bioreactors has been demonstrated to be an useful tool also in the treatment of vegetation waters from olive oil mills. |
| Databáze: | OpenAIRE |
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