Use of MALDI-TOF MS (Bruker Daltonics) for identification of Mycobacterium species isolated directly from liquid medium
| Autor: | Rebeca Fernández-Simon, María Luisa Monforte-Cirac, Marta Fernández-Esgueva, Blanca Fortuño, Ana Isabel López-Calleja, Jesús Viñuelas-Bayón |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Tuberculosis Diagnostic Tests Routine 030106 microbiology Nontuberculous Mycobacteria Mycobacterium tuberculosis Liquid medium Biology bacterial infections and mycoses biology.organism_classification medicine.disease Culture Media Microbiology 03 medical and health sciences Matrix-assisted laser desorption/ionization 0302 clinical medicine Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization medicine 030212 general & internal medicine Mycobacterium species |
| Zdroj: | Enfermedades infecciosas y microbiologia clinica (English ed.). 39:241-243 |
| ISSN: | 2529-993X |
| DOI: | 10.1016/j.eimce.2020.05.012 |
| Popis: | Objectives The aim of this study was to describe the evaluation of the use of MALDI-TOF MS for the identification of non-tuberculous mycobacteria (NTM) and Mycobacterium tuberculosis directly from liquid MGIT cultures from January 2017 to December 2017. Material/methods A total of 155 isolates (mainly respiratory) were analyzed by MALDI-TOF MS (Bruker Daltonics) directly from MGIT liquid medium with a previous extraction procedure. Results MALDI-TOF MS generated acceptable scores for 152 isolates (98.06%). Fifty isolates were identified as M. tuberculosis complex and the remaining 105 as NTM (M. abscessus subsp. abscessus, M. avium, M. celatum, M chelonae, M. chimaera, M. fortuitum, M. gordonae, M. intracellulare, M. kansasii, M. lentiflavum, M. mageritense, M. mucogenicum and M. xenopi). Conclusions These results indicate that MALDI-TOF MS can be useful to identify mycobacteria directly from MGIT cultures and is an accurate, rapid and cost-effective system to be used as a routine method. |
| Databáze: | OpenAIRE |
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