The barley transcription factor HvMYB1 is a positive regulator of drought tolerance
| Autor: | Peter Morris, Ross D. Alexander, Charlotte Wendelboe-Nelson |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Osmotic shock Physiology Drought tolerance Plant Science 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Plant Growth Regulators Gene Expression Regulation Plant Gene expression Genetics MYB Abscisic acid Plant Proteins chemistry.chemical_classification Dehydration biology Glutathione peroxidase fungi food and beverages Hordeum Plant Transpiration Plants Genetically Modified Cell biology 030104 developmental biology chemistry Catalase biology.protein Hordeum vulgare Abscisic Acid Transcription Factors 010606 plant biology & botany |
| Zdroj: | Plant Physiology and Biochemistry. 142:246-253 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2019.07.014 |
| Popis: | Transcription factors such as MYB have previously been associated with the plant response to drought. In this work, studies on the function of the barley (Hordeum vulgare L.) transcription factor HvMYB1 show that gene expression is upregulated in wildtype barley roots and leaves under drought and osmotic stress. Transgenic barley plants that overexpress HvMYB1 were found to be more resistant to drought, showing enhanced relative water content and reduced water loss rate and stomatal conductance as compared to control plants. Levels of the osmolyte proline were enhanced as was expression of dehydrin HvDNH6 in the transgenic lines under drought conditions. The levels of the reactive oxygen species H2O2 were enhanced in wildtype roots and leaves by drought, but less so in the HvMYB1 overexpressing lines. Enzyme activity of the low affinity H2O2 degrading enzyme catalase (EC 1.11.1.6) was also lower in droughted HvMYB1 overexpressing lines. Gene expression of the high affinity ROS scavengers ASCORBATE PEROXIDASE and GLUTATHIONE PEROXIDASE was found to be constitutively high in the overexpressing lines, whereas CATALASE gene expression was similar to the control plants. These results suggest a role for HvMYB1 in protecting plants against drought in the vegetative plant by acting as a mediator of abscisic acid action. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect