A novel nuclear protein interacts with the symbiotic DMI3 calcium- and calmodulin-dependent protein kinase of Medicago truncatula
| Autor: | Li Huey Yeun, Jeong-Hwan Mun, Elsa Messinese, Sebastian Schornack, Douglas R. Cook, Géraldine Lougnon, Pierre Rougé, Annick Barre, Jean-Jacques Bono, Jean-Michel Ané, Dhileepkumar Jayaraman |
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| Přispěvatelé: | Université Paris-Sud - Paris 11 (UP11), Unité mixte de recherche interactions plantes-microorganismes, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées |
| Rok vydání: | 2007 |
| Předmět: |
0106 biological sciences
Yellow fluorescent protein Models Molecular Root nodule Physiology [SDV]Life Sciences [q-bio] Green Fluorescent Proteins Molecular Sequence Data 01 natural sciences Conserved sequence Rhizobia Nod factor 03 medical and health sciences Mycorrhizae Two-Hybrid System Techniques Botany Medicago truncatula [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Amino Acid Sequence Nuclear protein Symbiosis ComputingMilieux_MISCELLANEOUS Conserved Sequence 030304 developmental biology Plant Proteins 0303 health sciences biology fungi food and beverages General Medicine 15. Life on land biology.organism_classification Cell biology Calmodulin dependent protein kinase [SDE]Environmental Sciences Calcium-Calmodulin-Dependent Protein Kinases biology.protein RNA Interference Root Nodules Plant Agronomy and Crop Science Sequence Alignment 010606 plant biology & botany |
| Zdroj: | Molecular Plant-Microbe Interactions Molecular Plant-Microbe Interactions, American Phytopathological Society, 2007, 20 (8), pp.912-921. ⟨10.1094/MPMI-20-8-0912⟩ |
| ISSN: | 0894-0282 |
| Popis: | Many higher plants establish symbiotic relationships with arbuscular mycorrhizal (AM) fungi that improve their ability to acquire nutrients from the soil. In addition to establishing AM symbiosis, legumes also enter into a nitrogen-fixing symbiosis with bacteria known as rhizobia that results in the formation of root nodules. Several genes involved in the perception and transduction of bacterial symbiotic signals named “Nod factors” have been cloned recently in model legumes through forward genetic approaches. Among them, DMI3(Doesn't Make Infections 3) is a calcium- and calmodulin-dependent kinase required for the establishment of both nodulation and AM symbiosis. We have identified, by a yeast two-hybrid system, a novel protein interacting with DMI3 named IPD3 (Interacting Protein of DMI3). IPD3 is predicted to interact with DMI3 through a C-terminal coiled-coil domain. Chimeric IPD3∷GFP is localized to the nucleus of transformed Medicago truncatula root cells, in which split yellow fluorescent protein assays suggest that IPD3 and DMI3 physically interact in Nicotiana benthamiana. Like DMI3, IPD3 is extremely well conserved among the angiosperms and is absent from Arabidopsis. Despite this high level of conservation, none of the homologous proteins have a demonstrated biological or biochemical function. This work provides the first evidence of the involvement of IPD3 in a nuclear interaction with DMI3. |
| Databáze: | OpenAIRE |
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