Comparative Transcriptome Analysis of Bombyx mori (Lepidoptera) Larval Midgut Response to BmNPV in Susceptible and Near-Isogenic Resistant Strains

Autor: Yan Ma, Lei Geng, Dong-Qiong Fei, Hai-Zhong Yu, Jia-Ping Xu, Dong Yu, Shang-Zhi Zhang, Xue-yang Wang
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Gene Expression
lcsh:Medicine
Apoptosis
Virus Replication
Biochemistry
01 natural sciences
Transcriptome
Larvae
lcsh:Science
Cytoskeleton
Genetics
Multidisciplinary
Cell Death
biology
Genomics
Insects
Cell Processes
Larva
Host-Pathogen Interactions
Insect Proteins
Cellular Structures and Organelles
Transcriptome Analysis
Research Article
Arthropoda
Microbiology
03 medical and health sciences
Immune system
Protein Domains
Immunity
Bombyx mori
Virology
Animals
Gene
Innate immune system
Metamorphosis
Gene Expression Profiling
lcsh:R
fungi
Organisms
Biology and Life Sciences
Computational Biology
Proteins
Molecular Sequence Annotation
Cell Biology
Genome Analysis
Bombyx
biology.organism_classification
Invertebrates
Viral Replication
Immunity
Innate
Nucleopolyhedroviruses
Gene expression profiling
Cytoskeletal Proteins
010602 entomology
Gene Ontology
030104 developmental biology
Gene Expression Regulation
Viral replication
Silkworms
lcsh:Q
Apoptosis Regulatory Proteins
Carrier Proteins
Developmental Biology
Zdroj: PLOS ONE
PLoS ONE, Vol 11, Iss 5, p e0155341 (2016)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0155341
Popis: Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the primary pathogens causing severe economic losses in sericulture. However, the molecular mechanism of silkworm resistance to BmNPV remains largely unknown. Here, the recurrent parent P50 (susceptible strain) and the near-isogenic line BC9 (resistance strain) were used in a comparative transcriptome study examining the response to infection with BmNPV. A total of 14,300 unigenes were obtained from two different resistant strains; of these, 869 differentially expressed genes (DEGs) were identified after comparing the four transcriptomes. Many DEGs associated with protein metabolism, cytoskeleton, and apoptosis may be involved in the host response to BmNPV infection. Moreover, some immunity related genes were also altered following BmNPV infection. Specifically, after removing genetic background and individual immune stress response genes, 22 genes were found to be potentially involved in repressing BmNPV infection. These genes were related to transport, virus replication, intracellular innate immune, and apoptosis. Our study provided an overview of the molecular mechanism of silkworm resistance to BmNPV infection and laid a foundation for controlling BmNPV in the future.
Databáze: OpenAIRE
Externí odkaz: