Screening archaeological bone for palaeogenetic and palaeoproteomic studies

Autor:	Victoria E. Mullin, Christina Papageorgopoulou, Matthew D. Teasdale, Henrik B. Hansen, Birgit Gehlen, Joachim Burger, Ashot Margaryan, Adamantios Sampson, Laura Winkelbach, Niels Lynnerup, Kirsty Penkman, Daniel G. Bradley, Amelie Scheu, Morten E. Allentoft, Martina Unterländer, Ioannis Liritzis, Matthew J. Collins, Ioannis Kontopoulos, Martin Street, Susanne Kreutzer
Přispěvatelé:	Kontopoulos, Ioannis [0000-0001-5591-8917], Kreutzer, Susanne [0000-0001-6286-534X], Gehlen, Birgit [0000-0003-1345-8072], Apollo - University of Cambridge Repository
Rok vydání:	2020
Předmět:	Proteomics Social Sciences Marine and Aquatic Sciences 01 natural sciences Biochemistry Spectroscopy Fourier Transform Infrared Limnology Screening method 0303 health sciences Multidisciplinary Ancient DNA Chemistry Fossils FOS: Social sciences Nucleic acids Archaeology Attenuated total reflection Medicine Physical Anthropology Organic content Research Article 010506 paleontology Science Infrared spectroscopy Paleoenvironments Bone and Bones 03 medical and health sciences Paleoanthropology Genetics Animals Humans Paleolimnology DNA Ancient Paleozoology 030304 developmental biology 0105 earth and related environmental sciences Ecology and Environmental Sciences Biology and Life Sciences Proteins Paleontology DNA Earth sciences Anthropology Paleobiology Paleogenetics Collagens
Zdroj:	PLoS ONE Kontopoulos, I, Penkman, K, Mullin, V E, Winkelbach, L, Unterländer, M, Scheu, A, Kreutzer, S, Hansen, H B, Margaryan, A, Teasdale, M D, Gehlen, B, Street, M, Lynnerup, N, Liritzis, I, Sampson, A, Papageorgopoulou, C, Allentoft, M E, Burger, J, Bradley, D G & Collins, M J 2020, ' Screening archaeological bone for palaeogenetic and palaeoproteomic studies ', PLoS ONE, vol. 15, no. 6, e0235146 . https://doi.org/10.1371/journal.pone.0235146 PLoS ONE, Vol 15, Iss 6, p e0235146 (2020)
ISSN:	1932-6203
DOI:	10.1371/journal.pone.0235146
Popis:	Funder: FP7 Ideas: European Research Council; funder-id: http://dx.doi.org/10.13039/100011199; Grant(s): 295729 The recovery and analysis of ancient DNA and protein from archaeological bone is time-consuming and expensive to carry out, while it involves the partial or complete destruction of valuable or rare specimens. The fields of palaeogenetic and palaeoproteomic research would benefit greatly from techniques that can assess the molecular quality prior to sampling. To be relevant, such screening methods should be effective, minimally-destructive, and rapid. This study reports results based on spectroscopic (Fourier-transform infrared spectroscopy in attenuated total reflectance [FTIR-ATR]; n = 266), palaeoproteomic (collagen content; n = 226), and palaeogenetic (endogenous DNA content; n = 88) techniques. We establish thresholds for three different FTIR indices, a) the infrared splitting factor [IRSF] that assesses relative changes in bioapatite crystals’ size and homogeneity; b) the carbonate-to-phosphate [C/P] ratio as a relative measure of carbonate content in bioapatite crystals; and c) the amide-to-phosphate ratio [Am/P] for assessing the relative organic content preserved in bone. These thresholds are both extremely reliable and easy to apply for the successful and rapid distinction between well- and poorly-preserved specimens. This is a milestone for choosing appropriate samples prior to genomic and collagen analyses, with important implications for biomolecular archaeology and palaeontology.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6401e568f26b46c7ab0a3f376c5efb90 https://pubmed.ncbi.nlm.nih.gov/32584871 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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