Study on the recognition of G-quadruplexes by two stereoisomers of alkaloids
| Autor: | Fangyuan Li, Lin Kang, Dan Guo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Future studies Isotetrandrine Stereochemistry 02 engineering and technology G-quadruplex Benzylisoquinolines 01 natural sciences Biochemistry Analytical Chemistry Proto-Oncogene Proteins c-myc chemistry.chemical_compound Alkaloids Molecule heterocyclic compounds Hydrogen bond Circular Dichroism 010401 analytical chemistry Hydrogen Bonding Stereoisomerism 021001 nanoscience & nanotechnology Ligand (biochemistry) 0104 chemical sciences Cancer treatment G-Quadruplexes Tetrandrine Proto-Oncogene Proteins c-bcl-2 chemistry Thermodynamics 0210 nano-technology |
| Zdroj: | Analytical and Bioanalytical Chemistry. 411:5555-5561 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-019-01937-5 |
| Popis: | G-quadruplexes have been widely researched as new targets for cancer treatment owing to their non-canonical structure and crucial role in biological processes. Although attention has been paid to the development of selective G-quadruplex ligands, few studies have focused on the binding affinity of stereoisomers towards G-quadruplex, which will be conducive to support the optimal design of G-quadruplex ligands in future studies. Here, tetrandrine and isotetrandrine were used to study the binding affinity and difference of stereoisomers towards G-quadruplex structures. The results showed that tetrandrine had a high possibility of binding to the N-myc and Bcl-2 G-quadruplexes through hydrogen bonding, whereas the possibility of binding of isotetrandrine was low and it seemed to have no possibility of forming hydrogen bonds. Our study shows that optical isomerism of ligand molecules has an important effect on G-quadruplex recognition, which is helpful for the design of G-quadruplex ligands in future studies. Graphical abstract. |
| Databáze: | OpenAIRE |
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