Molecular farming of antimicrobial peptides: available platforms and strategies for improving protein biosynthesis using modified virus vectors
| Autor: | Nicolau B. da Cunha, Octavio L. Franco, Kamila Botelho Sampaio, Simoni Campos Dias, Fabricio F. Costa, Michel Lopes Leite |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Magnifection Genetic Vectors Molecular Farming Antimicrobial peptides Heterologous Nicotiana benthamiana Context (language use) Computational biology Gene delivery Biology 01 natural sciences Viral vector transient expression 03 medical and health sciences antimicrobial peptides Tobacco Protein biosynthesis lcsh:Science Multidisciplinary Agrobacterium tumefaciens Plants Genetically Modified biology.organism_classification 030104 developmental biology Protein Biosynthesis lcsh:Q Antimicrobial Cationic Peptides Biotechnology 010606 plant biology & botany |
| Zdroj: | Anais da Academia Brasileira de Ciências, Issue: ahead, Published: 18 OCT 2018 Anais da Academia Brasileira de Ciências, Iss 0 (2018) Anais da Academia Brasileira de Ciências v.91 suppl.1 2019 Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências (ABC) instacron:ABC Anais da Academia Brasileira de Ciências, Volume: 91 Supplement 1, Article number: e20180124, Published: 18 OCT 2018 |
| Popis: | The constant demand for new antibiotic drugs has driven efforts by the scientific community to prospect for peptides with a broad spectrum of action. In this context, antimicrobial peptides (AMPs) have acquired great scientific importance in recent years due to their ability to possess antimicrobial and immunomodulatory activity. In the last two decades, plants have attracted the interest of the scientific community and industry as regards their potential as biofactories of heterologous proteins. One of the most promising approaches is the use of viral vectors to maximize the transient expression of drugs in the leaves of the plant Nicotiana benthamiana. Recently, the MagnifectionTM expression system was launched. This sophisticated commercial platform allows the assembly of the viral particle in leaf cells and the systemic spread of heterologous protein biosynthesis in green tissues caused by Agrobacterium tumefaciens “gene delivery method”. The system also presents increased gene expression levels mediated by potent viral expression machinery. These characteristics allow the mass recovery of heterologous proteins in the leaves of N. benthamiana in 8 to 10 days. This system was highly efficient for the synthesis of different classes of pharmacological proteins and contains enormous potential for the rapid and abundant biosynthesis of AMPs. |
| Databáze: | OpenAIRE |
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