Therapeutic Regeneration of Lymphatic and Immune Cell Functions upon Lympho-organoid Transplantation

Autor: Annapaola Andolfo, M.T. Palano, Sylvain Mukenge, Vincenzo Russo, Denise Drago, Luca Genovese, Antonello E. Spinelli, Elisa Lenti, Andrea Brendolan, Steven T. Proulx, Massimo Alfano, Silvia Bianchessi, Tatiana V. Petrova, Laura Raccosta
Rok vydání: 2019
Předmět:
0301 basic medicine
Stromal cell
610 Medicine & health
Mice
Transgenic
Biology
Biochemistry
Mice
03 medical and health sciences
0302 clinical medicine
Immune system
Report
Genetics
Animals
Regeneration
Progenitor cell
lcsh:QH301-705.5
lcsh:R5-920
Decellularization
Tissue Scaffolds
integumentary system
Cells
Immobilized/metabolism
Cells
Immobilized/transplantation
Extracellular Matrix/chemistry
Extracellular Matrix/transplantation
Lymph Nodes/metabolism
Lymph Nodes/transplantation
Organoids/metabolism
Organoids/transplantation
Tissue Scaffolds/chemistry
lymphatic system
lympho-organoids
lymphoid tissue development
preclinical mouse models
stromal cell biology
tissue engineering
tissue regeneration
Regeneration (biology)
Cell Biology
Cells
Immobilized
Extracellular Matrix
Organoids
Transplantation
030104 developmental biology
Lymphatic system
lcsh:Biology (General)
Cancer research
570 Life sciences
biology
Lymph Nodes
Lymph
lcsh:Medicine (General)
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Stem Cell Reports, 12 (6)
Stem cell reports, vol. 12, no. 6, pp. 1260-1268
Lenti, Elisa; Bianchessi, Silvia; Proulx, Steven Thomas; Palano, Maria Teresa; Genovese, Luca; Raccosta, Laura; Spinelli, Antonello; Drago, Denise; Andolfo, Annapaola; Alfano, Massimo; Petrova, Tatiana V; Mukenge, Sylvain; Russo, Vincenzo; Brendolan, Andrea (2019). Therapeutic Regeneration of Lymphatic and Immune Cell Functions upon Lympho-organoid Transplantation. Stem cell reports, 12(6), pp. 1260-1268. Elsevier 10.1016/j.stemcr.2019.04.021
Stem Cell Reports, Vol 12, Iss 6, Pp 1260-1268 (2019)
ISSN: 2213-6711
DOI: 10.1016/j.stemcr.2019.04.021
Popis: Lymph nodes (LNs) are secondary lymphoid tissues that play a critical role in filtering the lymph and promoting adaptive immune responses. Surgical resection of LNs, radiation therapy, or infections may damage lymphatic vasculature and compromise immune functions. Here, we describe the generation of functional synthetic lympho-organoids (LOs) using LN stromal progenitors and decellularized extracellular matrix-based scaffolds, two basic constituents of secondary lymphoid tissues. We show that upon transplantation at the site of resected LNs, LOs become integrated into the endogenous lymphatic vasculature and efficiently restore lymphatic drainage and perfusion. Upon immunization, LOs support the activation of antigen-specific immune responses, thus acquiring properties of native lymphoid tissues. These findings provide a proof-of-concept strategy for the development of functional lympho-organoids suitable for restoring lymphatic and immune cell functions.
Stem Cell Reports, 12 (6)
Databáze: OpenAIRE
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