Species differences in bacterial NhaA Na+/H+ exchangers
| Autor: | Eva Danner, Octavian Călinescu, Carola Hunte, Marc Böhm, Klaus Fendler |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Salmonella typhimurium
Salmonella Sodium-Hydrogen Exchangers Cation/proton antiport Kinetic analysis Biophysics lac operon medicine.disease_cause Biochemistry 03 medical and health sciences Bacterial Proteins Species Specificity Structural Biology NhaA ddc:570 Genetics medicine Escherichia coli Binding site Molecular Biology 030304 developmental biology 0303 health sciences biology Helicobacter pylori Chemistry 030302 biochemistry & molecular biology Transporter Cell Biology Hydrogen-Ion Concentration biology.organism_classification Electrophysiological Phenomena Kinetics Solid supported membrane Bacteria Homeostasis |
| Popis: | Bacteria have adapted their NhaA Na+/H+ exchangers responsible for salt homeostasis to their different habitats. We present an electrophysiological and kinetic analysis of NhaA from Helicobacter pylori and compare it to the previously investigated exchangers from Escherichia coli and Salmonella typhimurium. Properties of all three transporters are described by a simple model using a single binding site for H+ and Na+. We show that H. pylori NhaA only has a small acidic shift of its pH-dependent activity profile compared to the other transporters and discuss why a more drastic change in its pH activity profile is not physiologically required. |
| Databáze: | OpenAIRE |
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