Investigating cross-contamination by yeast strains from dental solid waste to waste-handling workers by DNA sequencing
| Autor: | Rodrigo Assuncao Holanda, Luiz de Macêdo Farias, Maria Auxiliadora Roque de Carvalho, Cristina Dutra Vieira, Paula Prazeres Magalhães, Simone Gonçalves dos Santos, Maria Aparecida Resende-Stoianoff, Thais Furtado Ferreira Magalhães, Thaysa Leite Tagliaferri |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Municipal solid waste Antifungal Agents 030106 microbiology 030231 tropical medicine Antifungal drug Microbial Sensitivity Tests Biology yeast Solid Waste Microbiology DNA sequencing 03 medical and health sciences 0302 clinical medicine Drug Resistance Fungal Humans Internal transcribed spacer DNA Fungal Candida Original Research Base Sequence business.industry Dental Waste UPGMA Genetic Variation Sequence Analysis DNA Contamination antifungal resistance Yeast Biotechnology Waste Disposal Facilities environmental microbiology health care solid waste DNA Intergenic fungal genes business |
| Zdroj: | MicrobiologyOpen |
| ISSN: | 2045-8827 |
| Popis: | Trying to widen the discussion on the risks associated with dental waste, this study proposed to investigate and genetically compare yeast isolates recovered from dental solid waste and waste workers. Three samples were collected from workers' hands, nasal mucosa, and professional clothing (days 0, 30, and 180), and two from dental waste (days 0 and 180). Slide culture, microscopy, antifungal drug susceptibility, intersimple sequence repeat analysis, and amplification and sequencing of internal transcribed spacer regions were performed. Yeast strains were recovered from all waste workers' sites, including professional clothes, and from waste. Antifungal susceptibility testing demonstrated that some yeast recovered from employees and waste exhibited nonsusceptible profiles. The dendrogram demonstrated the presence of three major clusters based on similarity matrix and UPGMA grouping method. Two branches displayed 100% similarity: three strains of Candida guilliermondii isolated from different employees, working in opposite work shifts, and from diverse sites grouped in one part of branch 1 and cluster 3 that included two samples of Candida albicans recovered from waste and the hand of one waste worker. The results suggested the possibility of cross‐contamination from dental waste to waste workers and reinforce the need of training programs focused on better waste management routines. |
| Databáze: | OpenAIRE |
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