Appropriate maturation and folding of 16S rRNA during 30S subunit biogenesis are critical for translational fidelity
| Autor: | Gloria M. Culver, Biswajoy Roy-Chaudhuri, Narayanaswamy Kirthi |
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| Rok vydání: | 2010 |
| Předmět: |
Models
Molecular Ribosomal Proteins Genetics Multidisciplinary Protein Conformation Eukaryotic Large Ribosomal Subunit Escherichia coli Proteins Mutation Missense Ribosome Subunits Small Bacterial Biological Sciences Ribosomal RNA Biology Ribosome 18S ribosomal RNA Acetyltransferases 23S ribosomal RNA Ribosomal protein Protein Biosynthesis RNA Ribosomal 16S Escherichia coli Nucleic Acid Conformation Eukaryotic Small Ribosomal Subunit Eukaryotic Ribosome |
| Zdroj: | Proceedings of the National Academy of Sciences. 107:4567-4572 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0912305107 |
| Popis: | Ribosomal protein S5 is critical for small ribosomal subunit (SSU) assembly and is indispensable for SSU function. Previously, we identified a point mutation in S5, (G28D) that alters both SSU formation and translational fidelity in vivo, which is unprecedented for other characterized S5 mutations. Surprisingly, additional copies of an extraribosomal assembly factor, RimJ, rescued all the phenotypes associated with S5(G28D), including fidelity defects, suggesting that the effect of RimJ on rescuing the miscoding of S5(G28D) is indirect. To understand the underlying mechanism, we focused on the biogenesis cascade and observed defects in processing of precursor 16S (p16S) rRNA in the S5(G28D) strain, which were rescued by RimJ. Analyses of p16S rRNA-containing ribosomes from other strains further supported a correspondence between the extent of 5 ′ end maturation of 16S rRNA and translational miscoding. Chemical probing of mutant ribosomes with additional leader sequences at the 5 ′ end of 16S rRNA compared to WT ribosomes revealed structural differences in the region of helix 1. Thus, the presence of additional nucleotides at the 5 ′ end of 16S rRNA could alter fidelity by changing the architecture of 16S rRNA in translating ribosomes and suggests that fidelity is governed by accuracy and completeness of the SSU biogenesis cascade. |
| Databáze: | OpenAIRE |
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