Production and Characterization of a Monoclonal Antibody Against the Ribosome Inactivating Protein Alpha Sarcin
| Autor: | Emanuela Tosi, Andrés JoséMaria Ferreri, Elena Ferraris di Celle, Maristella Caldera, Saturnino M. Muñoz, Stephanie Salvarelli, Silvana Canevari, Francisco P. Conde, Maria I. Colnaghi |
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| Rok vydání: | 1992 |
| Předmět: |
Antigens
Fungal Immunogen medicine.drug_class Immunology medicine.disease_cause Monoclonal antibody Epitope Fungal Proteins Epitopes Mice chemistry.chemical_compound Biosynthesis Endoribonucleases Genetics medicine Animals Protein Synthesis Inhibitors Hybridomas biology Toxin Immunotoxins Immunogenicity Ribosome-inactivating protein Antibodies Monoclonal Active site Molecular biology Aspergillus Biochemistry chemistry biology.protein Immunization Ribosomes |
| Zdroj: | Hybridoma. 11:437-446 |
| ISSN: | 0272-457X |
| DOI: | 10.1089/hyb.1992.11.437 |
| Popis: | In order to improve the purification of immunoconjugates containing alpha sarcin, a ribosome-inactivating protein, and in the attempt to define the enzymic region of the toxin, MAbs against alpha sarcin were produced. From 5 fusions, by adopting a short period of immunization and very low doses of the immunogen, 10 anti-toxin-producing clones were obtained. One of them, named MAsg2 (IgG2b), due to its specific reactivity and secreting properties, was selected for further characterization. MAsg2 was found to recognize an epitope which is common to two, i.e. alpha sarcin and clavatin, of the three different aspergillins tested, but is not involved in the active site of the toxins. |
| Databáze: | OpenAIRE |
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