Ethanol-Fixed, Paraffin-Embedded Tissue Imaging: Implications for Alzheimer’s Disease Research
| Autor: | Stephan B. H. Bach, George Perry, Savannah Dyer, Andrea R. Kelley, Madeline E. Colley, Xiongwei Zhu |
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| Rok vydání: | 2020 |
| Předmět: |
Tissue Fixation
Amyloid beta Computational biology Mass spectrometry imaging Alzheimer's disease research Alzheimer Disease Structural Biology Humans Senile plaques Process (anatomy) Spectroscopy Brain Chemistry Amyloid beta-Peptides Paraffin Embedding Ethanol biology Chemistry Optical Imaging Brain Proteins Intact protein Lipids Paraffin embedded tissue Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein Relevant information |
| Zdroj: | Journal of the American Society for Mass Spectrometry. 31:2416-2420 |
| ISSN: | 1879-1123 1044-0305 |
| DOI: | 10.1021/jasms.0c00195 |
| Popis: | Mass spectrometry imaging (MSI) is rapidly becoming a crucial tool in disease research. Fresh-frozen tissue is ideal for MSI because the protein and lipid structures are undisturbed by chemical fixatives; however, that means long-term preservation is limited. Formalin-fixed paraffin-embedded tissue has a virtually infinite shelf life, but whole proteins are difficult or impossible to image directly. To bridge this gap, we examine the use of ethanol-fixed, paraffin-embedded (EFPE) tissue for the localization of intact proteins and lipids and comment on implications in Alzheimer's disease (AD) research. The new sample preparation methods for EFPE tissues have allowed us to greatly broaden the information we can extract from MSI experiments. Our methods involve a xylene-free deparaffination for lipid analysis and an intact protein method for visualizing amyloid-beta plaques from human AD brain tissue. This unique combination streamlines the MSI sample preparation process while allowing for the most biologically and pathologically relevant information to be extracted from a single tissue source. |
| Databáze: | OpenAIRE |
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