Impact of a Single Point Mutation on the Antimicrobial and Fibrillogenic Properties of Cryptides from Human Apolipoprotein B
| Autor: | Rocco Di Girolamo, Giovanni Smaldone, Angela Arciello, Mariano Rumolo, Pompea Del Vecchio, Angela Cesaro, Eugenio Notomista, Rosa Gaglione, Rosario Oliva, Emilia Pedone, Maria De Luca, Luigi Petraccone |
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| Přispěvatelé: | Gaglione, R., Smaldone, G., Cesaro, A., Rumolo, M., De Luca, M., Di Girolamo, R., Petraccone, L., Del Vecchio, P., Oliva, R., Notomista, E., Pedone, E., Arciello, A. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Circular dichroism
bioactive cryptides medicine.drug_class Antibiotics single point mutation Pharmaceutical Science Peptide Article in vitro fibrillogenesis Pharmacy and materia medica Drug Discovery medicine chemistry.chemical_classification Point mutation In vitro fibrillogenesi Antimicrobial Phenotype RS1-441 Biochemistry chemistry anti-biofilm activity Molecular Medicine Medicine Bioactive cryptide Antibacterial activity Wound healing |
| Zdroj: | Pharmaceuticals, Vol 14, Iss 631, p 631 (2021) Pharmaceuticals Volume 14 Issue 7 |
| ISSN: | 1424-8247 |
| Popis: | Host defense peptides (HDPs) are gaining increasing interest, since they are endowed with multiple activities, are often effective on multidrug resistant bacteria and do not generally lead to the development of resistance phenotypes. Cryptic HDPs have been recently identified in human apolipoprotein B and found to be endowed with a broad-spectrum antimicrobial activity, with anti-biofilm, wound healing and immunomodulatory properties, and with the ability to synergistically act in combination with conventional antibiotics, while being not toxic for eukaryotic cells. Here, a multidisciplinary approach was used, including time killing curves, differential scanning calorimetry, circular dichroism, ThT binding assays, and transmission electron microscopy analyses. The effects of a single point mutation (Pro → Ala in position 7) on the biological properties of ApoB-derived peptide r(P)ApoBLPro have been evaluated. Although the two versions of the peptide share similar antimicrobial and anti-biofilm properties, only r(P)ApoBLAla peptide was found to exert bactericidal effects. Interestingly, antimicrobial activity of both peptide versions appears to be dependent from their interaction with specific components of bacterial surfaces, such as LPS or LTA, which induce peptides to form β-sheet-rich amyloid-like structures. Altogether, obtained data indicate a correlation between ApoB-derived peptides self-assembling state and their antibacterial activity. |
| Databáze: | OpenAIRE |
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