COVID-19 Seroprevalence among Healthcare Workers of a Large COVID-19 Hospital in Rome Reveals Strengths and Limits of Two Different Serological Tests
| Autor: | Michela Cicconi, Brunella Posteraro, Federica Foti, Maria Elena D'Alfonso, Domenico Staiti, Floriana D'Ambrosio, Fabio De-Giorgio, Stefania Boccia, Marcello Di Pumpo, E Carini, Filippo Berloco, Rosalba Ricci, Umberto Moscato, Roberta Pastorino, Domenico Pascucci, Maurizio Zega, Francesco Maria De Simone, Paola Cattani, Andrea Cambieri, Daniele Ignazio La Milia, Patrizia Laurenti, Gennaro Capalbo, Giuseppe Vetrugno, Gianfranco Damiani, Maurizio Sanguinetti, Massimo Fantoni, Francesco Castrini |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
serological tests
medicine.medical_specialty Coronavirus disease 2019 (COVID-19) Health Toxicology and Mutagenesis Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Health Personnel Rome lcsh:Medicine Test sensitivity Antibodies Viral Article Antibodies Serology 03 medical and health sciences 0302 clinical medicine Seroepidemiologic Studies Internal medicine medicine Seroprevalence Humans Serologic Tests 030212 general & internal medicine Viral Settore MED/42 - IGIENE GENERALE E APPLICATA 030304 developmental biology 0303 health sciences seroprevalence business.industry healthcare workers SARS-CoV-2 lcsh:R Public Health Environmental and Occupational Health COVID-19 Venous blood Gold standard (test) Confidence interval Hospitals point-of-care business |
| Zdroj: | International Journal of Environmental Research and Public Health Volume 18 Issue 5 International Journal of Environmental Research and Public Health, Vol 18, Iss 2650, p 2650 (2021) |
| ISSN: | 1660-4601 |
| DOI: | 10.3390/ijerph18052650 |
| Popis: | Healthcare workers are at the forefront against COVID-19, worldwide. Since Fondazione Policlinico Universitario A. Gemelli (FPG) IRCCS was enlisted as a COVID-19 hospital, the healthcare workers deployed to COVID-19 wards were separated from those with limited/no exposure, whereas the administrative staff were designated to work from home. Between 4 June and 3 July 2020, an investigation was conducted to evaluate the seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulin (IgG) antibodies among the employees of the FPG using point-of-care (POC) and venous blood tests. Sensitivity, specificity, and predictive values were determined with reverse-transcription polymerase chain reaction on nasal/oropharyngeal swabs as the diagnostic gold standard. The participants enrolled amounted to 4777. Seroprevalence was 3.66% using the POC test and 1.19% using the venous blood test, with a significant difference (p < 0.05). The POC test sensitivity and specificity were, respectively, 63.64% (95% confidence interval (CI): 62.20% to 65.04%) and 96.64% (95% CI: 96.05% to 97.13%), while those of the venous blood test were, respectively, 78.79% (95% CI: 77.58% to 79.94%) and 99.36% (95% CI: 99.07% to 99.55%). Among the low-risk populations, the POC test’s predictive values were 58.33% (positive) and 98.23% (negative), whereas those of the venous blood test were 92.86% (positive) and 98.53% (negative). According to our study, these serological tests cannot be a valid alternative to diagnose COVID-19 infection in progress. |
| Databáze: | OpenAIRE |
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