In vivo visualization of gold-loaded cells in mice using x-ray computed tomography
| Autor: | Giuliana Tromba, Bernhard H.J. Juurlink, Chris Hall, Elisabeth Schültke, Marco Stebel, Alberto Astolfo, Fulvia Arfelli, Robert D. Kirch, Davide Barbetta, Ralf Hendrik Menk, Laura-Adela Harsan |
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| Přispěvatelé: | Astolfo, Alberto, Elisabeth, Schültke, Ralf Hendrik, Menk, Robert D., Kirch, Bernhard H. J., Juurlink, Christopher, Hall, Laura Adela, Harsan, Marco, Stebel, Davide, Barbetta, Giuliana, Tromba, Arfelli, Fulvia |
| Rok vydání: | 2011 |
| Předmět: |
Male
Biodistribution Materials science Gold nanoparticle Monte Carlo method Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) High-resolution imaging Metal Nanoparticles Mice Nude Bioengineering Mice X ray computed In vivo Cell Line Tumor Gold nanoparticles Animals General Materials Science X-ray computed tomography Synchrotron radiation business.industry Brain Neoplasms Radiation dose Brain Rats in vivo Colloidal gold Molecular Medicine Tomography Gold Nuclear medicine business Tomography X-Ray Computed Monte Carlo Method Ex vivo Biomedical engineering |
| Zdroj: | Nanomedicine : nanotechnology, biology, and medicine. 9(2) |
| ISSN: | 1549-9642 |
| Popis: | The ability to perform cell tracking using x-ray computed tomography combined with gold nanoparticles has been demonstrated recently on ex vivo samples using different malignant and nonmalignant cell lines. Here we proved the concept of the method for in vivo assessment in a small-animal model of malignant brain tumors. The limitations of the method due to radiation dose constraints were investigated using Monte Carlo simulations. Taking into consideration different x-ray entrance doses and the spatial resolution, the visibility of the cell clusters was evaluated. The results of the experiments conducted on mice implanted with F98 tumor cells confirmed the prediction of the Monte Carlo calculations. Small clusters of cells exogenously loaded with gold nanoparticles could be visualized using our in vivo method.This article discusses the use of CT-based detection of gold nanoparticle loaded cells of interest in small-animal models of malignant brain tumors, where small clusters of cells loaded with gold nanoparticles could be visualized. |
| Databáze: | OpenAIRE |
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