| Autor: |
Stephen, Tottey, Yoko, Shoji, R, Mark Jones, Konstantin, Musiychuk, Jessica A, Chichester, Kazutoyo, Miura, Luwen, Zhou, Shwu-Maan, Lee, Jordan, Plieskatt, Yimin, Wu, Carole A, Long, Stephen J, Streatfield, Vidadi, Yusibov |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Vaccine. 41:938-944 |
| ISSN: |
0264-410X |
| Popis: |
Malaria kills around 409,000 people a year, mostly children under the age of five. Malaria transmission-blocking vaccines work to reduce malaria prevalence in a community and have the potential to be part of a multifaceted approach required to eliminate the parasites causing the disease. Pfs25 is a leading malaria transmission-blocking antigen and has been successfully produced in a plant expression system as both a subunit vaccine and as a virus-like particle. This study demonstrates an improved version of the virus-like particle antigen display molecule by eliminating known protease sites from the prior A85 variant. This re-engineered molecule, termed B29, displays three times the number of Pfs25 antigens per virus-like particle compared to the original Pfs25 virus-like particle. An improved purification scheme was also developed, resulting in a substantially higher yield and improved purity. The molecule was evaluated in a mouse model and found to induce improved transmission-blocking activity at lower doses and longer durations than the original molecule. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect