Hepatitis C Virus–Specific CD4+T Cell Response after Liver Transplantation Occurs Early, Is Multispecific, Compartmentalizes to the Liver, and Does Not Correlate with Recurrent Disease
| Autor: | Norbert H. Gruener, Reinhart Zachoval, Michael Houghton, Joern Tilman Gerlach, Maria-Christina Jung, Horst-Guenter Rau, Gustavo Baretton, Thomas Worzfeld, Gerd R. Pape, Maxim Mamin, Carl Albrecht Schirren |
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| Rok vydání: | 2001 |
| Předmět: |
CD4-Positive T-Lymphocytes
Graft Rejection Male Time Factors Genotype Biopsy Hepatitis C virus medicine.medical_treatment T cell Cell Culture Techniques Enzyme-Linked Immunosorbent Assay Hepacivirus Liver transplantation Biology medicine.disease_cause Cell Line Interferon-gamma Liver Function Tests Antigen Recurrence Interferon medicine Humans Immunology and Allergy medicine.diagnostic_test Hepatitis C Hepatitis C Antibodies Middle Aged medicine.disease Liver Transplantation Transplantation Infectious Diseases medicine.anatomical_structure Liver Antibody Formation Immunology RNA Viral Female Liver function tests Liver Failure medicine.drug |
| Zdroj: | The Journal of Infectious Diseases. 183:1187-1194 |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | The role of hepatitis C virus (HCV)-specific CD4+ T cells in recurrent HCV infection after orthotopic liver transplantation (OLTx) is unclear. In parallel, 73 intrahepatic and 73 blood-derived T cell lines were established from 34 patients. At a single cell level, virus-specific interferon (IFN)-gamma production to various HCV proteins was determined by ELISPOT assay: 45 (62%) of 73 liver- or blood-derived T cell lines produced IFN-gamma in response to one of the HCV antigens. HCV specificity was detected mainly in the liver (47% vs. 23% in the blood; P.05, chi(2) test) and was detectable earlier (or =6 months) significantly more often than later (6 months) after OLTx (78% vs 49%; P.05, chi(2) test). Histology, histologic activity index, liver enzymes, and virus load did not correlate with the occurrence of HCV-specific CD4+ T cells. Despite strong immunosuppressive treatment, OLTx recipients can develop an early, multispecific, preferentially intrahepatic CD4+ T cell response that decreases over time, making it a potential candidate target for novel therapeutic approaches in the transplant setting. |
| Databáze: | OpenAIRE |
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