Phototoxicity of flavoprotein miniSOG induced by bioluminescence resonance energy transfer in genetically encoded system NanoLuc-miniSOG is comparable with its LED-excited phototoxicity
| Autor: | E. I. Shramova, Anastasiya V. Ryabova, Sergey M. Deyev, O N Shilova, G. M. Proshkina |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Light Recombinant Fusion Proteins medicine.medical_treatment Biophysics Apoptosis Photodynamic therapy DNA Fragmentation 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Fluorescence Resonance Energy Transfer medicine Humans Bioluminescence Radiology Nuclear Medicine and imaging Luciferase Photosensitizer Luciferases Radiation Flavoproteins Radiological and Ultrasound Technology Chemistry Fusion protein Nanostructures Light intensity 030104 developmental biology 030220 oncology & carcinogenesis Cancer cell Reactive Oxygen Species Phototoxicity |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 188:107-115 |
| ISSN: | 1011-1344 |
| DOI: | 10.1016/j.jphotobiol.2018.09.006 |
| Popis: | Photodynamic therapy (PDT) is a clinical, minimally invasive method for destroying cancer cells in the presence of a photosensitizer, oxygen, and a light source. The main obstacle for the PDT treatment of deep tumors is a strong reduction of the excitation light intensity as a result of its refraction, reflection, and absorption by biological tissues. Internal light sources based on bioluminescence resonance energy transfer can be a solution of this problem. Here we show that luciferase NanoLuc being expressed as a fusion protein with phototoxic flavoprotein miniSOG in cancer cells in the presence of furimazine (highly specific NanoLuc substrate) induces a photodynamic effect of miniSOG comparable with its LED-excited (Light Emitting Diode) phototoxicity. Luminescence systems based on furimazine and hybrid protein NanoLuc-miniSOG targeted to mitochondria or cellular membranes possess the similar energy transfer efficiencies and similar BRET-induced cytotoxic effects on cancer cells, though the mechanisms of BRET-induced cell death are different. As the main components of the proposed system for BRET-mediated PDT are genetically encoded (luciferase and phototoxic protein), this system can potentially be delivered to any site in the organism and thus may be considered as a promising approach for simultaneous delivery of light source and photosensitizer in deep-lying tumors and metastasis anywhere in the body. |
| Databáze: | OpenAIRE |
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