A clonal stem cell line established from a mouse mammary placode with ability to generate functional mammary glands
| Autor: | Ruka Miyake, Yurika Sakai, Yasuhiro Tomooka, Teruyo Sakakura, Tadaaki Nakajima, Tatsuya Shimizu |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell Population Mammary gland Gene Expression Mice Inbred Strains Biology Cell Line 03 medical and health sciences Mammary Glands Animal 0302 clinical medicine Parenchyma medicine Animals Progenitor cell education Cells Cultured Mice Inbred BALB C education.field_of_study Stem Cells Embryo Cell Biology General Medicine Cell biology 030104 developmental biology medicine.anatomical_structure Adipose Tissue Cell culture 030220 oncology & carcinogenesis Female Tumor Suppressor Protein p53 Stem cell Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology - Animal. 55:861-871 |
| ISSN: | 1543-706X 1071-2690 |
| DOI: | 10.1007/s11626-019-00406-8 |
| Popis: | The mammary gland develops from the placode at ectodermal invagination. The rudimentary parenchyma (mammary bud) develops mammary trees and alveolar structures, suggesting that the mammary bud consists of stem/progenitor cells. Here, we established a clonal stem cell line from a mammary bud of a p53 null female embryo at day 14.5. FP5-3-1 line was a homogeneous cell population with polygonal epithelial morphology and spontaneously became heterogeneous during passages. Recloning gave rise to four sublines; three sublines have basal epithelial property and one subline has luminal epithelial property. The former sublines generate functional mammary glands when injected into cleared fat pads and the latter subline does not. The cell lines also express many stemness-related genes. The clonal cell lines established in the present study are shown to be mammary stem cells and not tumorigenic. They provide useful models for normal and tumor biology of the mammary gland in vivo and in vitro. |
| Databáze: | OpenAIRE |
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