Brain-derived neurotrophic factor-immunoreactive primary sensory neurons in the rat trigeminal ganglion and trigeminal sensory nuclei
| Autor: | Teruko Takano-Yamamoto, Hiroshi Kamioka, H.W. Jin, Ryuji Terayama, Toru Deguchi, Tomosada Sugimoto, Toshinori Yabuuchi, T. Yamaai, Hiroyuki Ichikawa |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Pathology medicine.medical_specialty Calcitonin Gene-Related Peptide TRPV Cation Channels Cell Count Sensory system Calcitonin gene-related peptide Trigeminal Nuclei Rats Sprague-Dawley Trigeminal ganglion Neurotrophic factors medicine Animals Neurons Afferent Axon Molecular Biology Dental Pulp Cell Size Brain-derived neurotrophic factor Chemistry Brain-Derived Neurotrophic Factor General Neuroscience Immunohistochemistry Retrograde tracing Sensory neuron Rats medicine.anatomical_structure Trigeminal Ganglion nervous system Neurology (clinical) Neuroscience Developmental Biology |
| Zdroj: | Brain Research. 1081:113-118 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2006.01.027 |
| Popis: | Immunohistochemistry for brain-derived neurotrophic factor (BDNF) was performed on the rat trigeminal ganglion (TG). The immunoreactivity (IR) was detected in 46% of TG neurons. These neurons were mostly small- or medium-sized (range, 149.7-1246.3 microm2; mean +/- SD = 373.4 +/- 151.6 microm2). A double immunofluorescence method also revealed that 54% of BDNF-immunoreactive (IR) neurons were immunoreactive for calcitonin-gene-related peptide. In addition, 93% of BDNF-IR TG neurons contained vanilloid receptor subtype 1. However, the co-expression of BDNF and vanilloid receptor 1-like receptor was very rare (less than 1%). In the trigeminal sensory nuclei, laminae II of the medullary dorsal horn was abundant in presumed BDNF-IR axon terminals. Such profiles were also detected in the dorsolateral part of the subnucleus oralis. The retrograde tracing and immunohistochemical methods demonstrated that BDNF-IR was common among cutaneous TG neurons (47%) but not tooth pulp TG neurons (13%). The present study indicates that BDNF-IR TG neurons have unmyelinated axons and project to the superficial medullary dorsal horn. It is likely that BDNF-containing neurons in both the trigeminal and spinal sensory systems have similarities in morphology and function. However, the content of BDNF in TG neurons probably depends on their peripheral targets. BDNF seems to convey nociceptive cutaneous input to the trigeminal sensory nuclei. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect