Development An Amperometric Microbial‐enzyme Hybrid Cholesterol Biosensor Based On Ionic Liquid MWCNT Carbon Paste Electrode

Autor:	İhsan Yaşa, Erhan Canbay, Erol Akyilmaz
Rok vydání:	2021
Předmět:	Microbial Biosensor Chromatography Chemistry Cholesterol MWCNT Cells Esterase Ionic Liquid Amperometry Analytical Chemistry Carbon paste electrode chemistry.chemical_compound Glucose Ionic liquid Microbial enzymes Electrochemistry Oxidase Biosensor
Zdroj:	Electroanalysis. 33:2381-2391
ISSN:	1521-4109 1040-0397
Popis:	In this study we report the development of an amperometric cholesterol biosensor based on cholesterol oxidase from Pseudomonas sp. and catalase immobilized in carbon paste electrode (CPE) modified with multiwall carbon nanotubes (MWCNT) and ionic liquid (IL). The working electrode (CPE/MWCNT-IL/Microorganism (MO)-Catalase) was characterized by impedance spectroscopy and cyclic voltammetry at different stages of its construction. This proposed cholesterol biosensor performed linear relationship in the range of 5-600 mu M with a low detection limit of 1.52 mu M. The biosensor showed good sensitivity and high selectivity and it was successfully applied for the measurement of cholesterol levels in lyophilized serum samples. Ege University Research Fund [15 FEN 021] This project was funded by the Ege University Research Fund (project 15 FEN 021).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63e6e516b5a50bdd818a708a5461cb3e https://doi.org/10.1002/elan.202100251 Zobrazit plný text záznamu Plný text ve formátu PDF