Dynamic changes in CD45RA−Foxp3high regulatory T-cells in chronic hepatitis C patients during antiviral therapy
| Autor: | Dongli Yue, Jianbin Li, Zhiqin Li, Yu Ping, Meng Wang, Xuezhong Shi, Yi Zhang, Zujiang Yu, Zhen Zhang, Bin Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Microbiology (medical) Alpha interferon chemical and pharmacologic phenomena Antiviral Agents T-Lymphocytes Regulatory HCV antiviral therapy Peripheral blood mononuclear cell Immunophenotyping lcsh:Infectious and parasitic diseases Flow cytometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ribavirin medicine Humans lcsh:RC109-216 IFN-α medicine.diagnostic_test biology business.industry Interferon-alpha FOXP3 Forkhead Transcription Factors hemic and immune systems General Medicine Transforming growth factor beta Hepatitis C Chronic Middle Aged In vitro 030104 developmental biology Infectious Diseases chemistry Immunology biology.protein Leukocyte Common Antigens Female business Treg cells 030215 immunology |
| Zdroj: | International Journal of Infectious Diseases, Vol 45, Iss C, Pp 5-12 (2016) |
| ISSN: | 1201-9712 |
| Popis: | Summary Objectives CD4 + Foxp3 + regulatory T-cells (Treg) are known to accumulate under certain pathological conditions. This study was conducted to evaluate the characteristics of and dynamic changes in Treg cells in chronic hepatitis C (CHC) patients during antiviral therapy. Methods One hundred and forty-five subjects were enrolled in this study, including 105 CHC patients and 40 healthy donors. The phenotypes and functions of Treg cells were analyzed by flow cytometry. Results A significant elevation in Treg cells was observed in the peripheral blood of CHC patients compared with healthy donors. Interestingly, compared with non-suppressive Treg (non-Treg) and resting Treg (rTreg) cells, activated Treg (aTreg) cells expressed higher levels of ectonucleotidase, CD39, and CD73. After treatment with interferon alpha (IFN-α) and ribavirin (RBV) in vitro, the frequencies of total Treg cells and aTreg cells in peripheral blood mononuclear cells (PBMC), as well as the levels of transforming growth factor beta (TGF-β) secreted by aTreg and non-Treg cells, were significantly decreased. Importantly, it was found that levels of aTreg cells in patients with a sustained virological response (SVR) were lower than in relapsed patients, suggesting that a high frequency of aTreg cells might be associated with a poor clinical outcome in HCV infection. Conclusion These results demonstrate a decreasing trend in aTreg cells, which express higher levels of CD39, CD73, and TGF-β, in SVR patients during antiviral therapy. |
| Databáze: | OpenAIRE |
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