Membrane prestin expression correlates with the magnitude of prestin-associated charge movement
| Autor: | William E. Brownell, Guillaume Duret, Haiying Liu, Fred A. Pereira, Michelle L. Seymour, Matthew J. Volk, Lavanya Rajagopalan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Patch-Clamp Techniques Time Factors Anion Transport Proteins Analytical chemistry Electric Capacitance Article Membrane Potentials 03 medical and health sciences Mice 0302 clinical medicine Animals Humans Inducer Cochlear Outer Hair Cells Prestin Ion Transport biology Chemistry Cell Membrane Charge density Charge (physics) Sensory Systems Cochlea Electrophysiology Mice Inbred C57BL Hair Cells Auditory Outer 030104 developmental biology Membrane HEK293 Cells Gene Expression Regulation Nonlinear Dynamics Sulfate Transporters Doxycycline biology.protein Biophysics sense organs Lateral wall Gerbillinae 030217 neurology & neurosurgery |
| Zdroj: | Hearing research. 339 |
| ISSN: | 1878-5891 |
| Popis: | Full expression of electromotility, generation of non-linear capacitance (NLC), and high-acuity mammalian hearing require prestin function in the lateral wall of cochlear outer hair cells (OHCs). Estimates of the number of prestin molecules in the OHC membrane vary, and a consensus has not emerged about the correlation between prestin expression and prestin-associated charge movement in the OHC. Using an inducible prestin-expressing cell line, we demonstrate that the charge density, but not the voltage at peak capacitance, directly correlates with the amount of prestin in the plasma membrane. This correlation is evident in studies involving a controlled increase of prestin expression with time after induction and inducer dose-response. Conversely, membrane prestin levels and charge density gradually decline together following the reduction of prestin levels from a steady state by removal of the inducer. Thus, charge density directly correlates with the level of membrane prestin expression, whereas changing membrane levels of prestin have no effect on the voltage at peak capacitance in this inducible prestin-expressing cell line. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect