Intracellular pH sensing is altered by plasma membrane PIP aquaporin co-expression
| Autor: | Gabriela Amodeo, Karina Alleva, Cintia Jozefkowicz, Gabriela Soto, Victoria Andrea Vitali, Jorge Bellati |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Intracellular Fluid
Whole membrane Cell Membrane Permeability DNA Plant Intracellular pH Molecular Sequence Data Gene Expression Aquaporin Plant Science Gating In Vitro Techniques Biology Aquaporins Genes Plant Plant Roots AQUAPORIN GATING Ciencias Biológicas Xenopus laevis Genetics Animals Amino Acid Sequence pH SENSING Phylogeny DNA Primers Plant Proteins Water transport Base Sequence Sequence Homology Amino Acid PLASMA MEMBRANE INTRINSIC PROTEIN CYTOSOLIC ACIDIFICATION Water General Medicine Hydrogen-Ion Concentration Permeation Biofísica Recombinant Proteins Cytosol Membrane Biochemistry Oocytes Biophysics Female Beta vulgaris Agronomy and Crop Science CIENCIAS NATURALES Y EXACTAS |
| DOI: | 10.1007/s11103-010-9658-8 |
| Popis: | The plant plasma membrane barrier can express aquaporins (PIP1 and PIP2) that show two intriguing aspects: (1) the potential of modulating whole membrane water permeability by co-expression of both types, which have recently been distinguished for showing a different capacity to reach the plasma membrane; and (2) the faculty to reduce water permeation through the pore after cytosolic acidification, as a consequence of a gating process. Our working hypothesis is that these two key features might enhance plasticity of the membrane water transport capacity if they jointly trigger any cooperative interaction. In previous work, we proved by biophysical approaches that the plasma membrane of the halophyte Beta vulgaris storage root presents highly permeable aquaporins that can be shut down by acidic pH. Root Beta vulgaris PIPs were therefore subcloned and expressed in Xenopus oocytes. Co-expression of BvPIP1;1 and BvPIP2;2 not only enhances oocyte plasma membrane water permeability synergistically but also reinforces pH inhibitory response from partial to complete shut down after cytosolic pH acidifi- cation. This pH dependent behavior shows that PIP1–PIP2 co-expression accounts for a different pH sensitivity in comparison with PIP2 expression. These results prove for the first time that PIP co-expression modulates the membrane water permeability through a pH regulatory response, enhancing in this way membrane versatility to adjust its water transfer capacity. Fil: Bellati, Jorge. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alleva, Karina Edith. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular "dr. Hector N Torres"; Argentina Fil: Vitali, Victoria Andrea. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Jozefkowicz, Cintia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Amodeo, Gabriela. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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