Expansions of tumor-reactive Vdelta1 gamma-delta T cells in newly diagnosed patients with chronic myeloid leukemia

Autor: Andrea Knight, Martin Piskacek, Michal Jurajda, Jirina Prochazkova, Zdenek Racil, Daniela Zackova, Jiri Mayer
Rok vydání: 2022
Předmět:
Zdroj: Cancer Immunology, Immunotherapy. 72:1209-1224
ISSN: 1432-0851
0340-7004
DOI: 10.1007/s00262-022-03312-3
Popis: Recent studies have underscored the importance of gamma-delta (γδ) T cells in mediating potent MHC-unrestricted cytotoxicity in numerous malignancies. Here, we analyzed Vδ1 and Vδ2 γδ T cell subsets in newly diagnosed chronic myeloid leukemia (CML) patients (n = 40) who had initiated tyrosine kinase inhibitor (TKI) therapy including imatinib (n = 22), nilotinib (n = 14) and dasatinib (n = 4). Patient peripheral blood samples were analyzed at diagnosis and monitored prospectively at 3, 6, 12 and 18 months post-TKI. γδ T cells isolated from healthy donors and CML patients were used against K562, LAMA-84 and KYO-1 cell lines and against primary CML cells in cytotoxicity assays. We found large expansions of Vδ1 and Vδ2 T cells in patients at diagnosis compared to age-matched healthy donors (n = 40) (p
Databáze: OpenAIRE
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