Discovery of Three Toxic Proteins of Klebsiella Phage fHe-Kpn01

Autor: Cindy M. Spruit, Xing Wan, Maria Pajunen, Anu Wicklund, Mikael Skurnik
Přispěvatelé: Department of Microbiology, Department of Bacteriology and Immunology, Medicum, Helsinki One Health (HOH), HUSLAB, Glycoscience Group, Biosciences
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
MECHANISM
antibiotic resistance
Capsule type
Antibiotic resistance
medicine.medical_treatment
Klebsiella pneumoniae
lcsh:QR1-502
medicine.disease_cause
toxic proteins
Genome
lcsh:Microbiology
Podoviridae
Bacteriophage
bacteriophage
Antibiotics
Drulisvirus
Genetics
biology
3. Good health
Virology & Molecular Biology
Infectious Diseases
Lytic cycle
ESCHERICHIA-COLI
COMPLEXES
CCA-ADDING ENZYME
Hypothetical proteins of unknown function
EXPRESSION
Phage therapy
030106 microbiology
TRANSFER-RNA
Virulence
CLASSIFICATION
03 medical and health sciences
Virology
SURVEILLANCE
medicine
Gene
Escherichia coli
IDENTIFICATION
DNA
biology.organism_classification
hypothetical proteins of unknown function
Virologie & Moleculaire Biologie
030104 developmental biology
Toxic proteins
1182 Biochemistry
cell and molecular biology
Zdroj: Viruses, Vol 12, Iss 544, p 544 (2020)
Viruses
Volume 12
Issue 5
Viruses, 12(5)
Viruses 12 (2020) 5
ISSN: 1999-4915
Popis: The lytic phage, fHe-Kpn01 was isolated from sewage water using an extended-spectrum beta-lactamase-producing strain of Klebsiella pneumoniae as a host. The genome is 43,329 bp in size and contains direct terminal repeats of 222 bp. The genome contains 56 predicted genes, of which proteomics analysis detected 29 different proteins in purified phage particles. Comparison of fHe-Kpn01 to other phages, both morphologically and genetically, indicated that the phage belongs to the family Podoviridae and genus Drulisvirus. Because fHe-Kpn01 is strictly lytic and does not carry any known resistance or virulence genes, it is suitable for phage therapy. It has, however, a narrow host range since it infected only three of the 72 tested K. pneumoniae strains, two of which were of capsule type KL62. After annotation of the predicted genes based on the similarity to genes of known function and proteomics results on the virion-associated proteins, 22 gene products remained annotated as hypothetical proteins of unknown function (HPUF). These fHe-Kpn01 HPUFs were screened for their toxicity in Escherichia coli. Three of the HPUFs, encoded by the genes g10, g22, and g38, were confirmed to be toxic.
Databáze: OpenAIRE
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