PEG-modified gadolinium nanoparticles as contrast agents for in vivo micro-CT
| Autor: | P. Joy Dunmore-Buyze, Maria Drangova, Eric Grolman, Elizabeth R. Gillies, David W. Holdsworth, Charmainne Cruje |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Imaging techniques and agents Pathology medicine.medical_specialty Gadolinium Science Contrast Media chemistry.chemical_element Spleen Article Polyethylene Glycols Myoblasts Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo PEG ratio medicine Animals Tissue Distribution Whole Body Imaging Colloids Cytotoxicity 030304 developmental biology 0303 health sciences Multidisciplinary Soft tissue X-Ray Microtomography Cardiovascular biology 3. Good health Mice Inbred C57BL medicine.anatomical_structure chemistry Preclinical research 030220 oncology & carcinogenesis Nanoparticles Medicine Biomedical engineering Ethylene glycol Quantitative analysis (chemistry) |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Vascular research is largely performed in rodents with the goal of developing treatments for human disease. Micro-computed tomography (micro-CT) provides non-destructive three-dimensional imaging that can be used to study the vasculature of rodents. However, to distinguish vasculature from other soft tissues, long-circulating contrast agents are required. In this study, we demonstrated that poly(ethylene glycol) (PEG)-coated gadolinium nanoparticles can be used as a vascular contrast agent in micro-CT. The coated particles could be lyophilized and then redispersed in an aqueous solution to achieve 100 mg/mL of gadolinium. After an intravenous injection of the contrast agent into mice, micro-CT scans showed blood pool contrast enhancements of at least 200 HU for 30 min. Imaging and quantitative analysis of gadolinium in tissues showed the presence of contrast agent in clearance organs including the liver and spleen and very low amounts in other organs. In vitro cell culture experiments, subcutaneous injections, and analysis of mouse body weight suggested that the agents exhibited low toxicity. Histological analysis of tissues 5 days after injection of the contrast agent showed cytotoxicity in the spleen, but no abnormalities were observed in the liver, lungs, kidneys, and bladder. |
| Databáze: | OpenAIRE |
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