Differential stimulatory and inhibitory effects of interleukin 4 on granulocytic and monocytic colony formation in human bone marrow cultures
| Autor: | J. H. Jansen, Roelof Willemze, G. J. H. M. Wientjens, Willem E. Fibbe, J. C. Kluin-Nelemans |
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| Rok vydání: | 1991 |
| Předmět: |
Macrophage colony-stimulating factor
medicine.medical_specialty medicine.medical_treatment Granulocyte Biology Monocytes Bone Marrow Internal medicine Granulocyte Colony-Stimulating Factor medicine Humans Cells Cultured Interleukin 3 Monocyte Growth factor Macrophage Colony-Stimulating Factor Interleukin Cell Biology Hematopoietic Stem Cells Molecular biology Recombinant Proteins Haematopoiesis Endocrinology medicine.anatomical_structure Interleukin-3 Bone marrow Interleukin-4 Interleukin-5 Cell Division Granulocytes |
| Zdroj: | International journal of cell cloning. 9(6) |
| ISSN: | 0737-1454 |
| Popis: | Both stimulatory and inhibitory effects of interleukin (IL) 4 on myelo-poiesis have been described. In this paper we further define the specificity of these effects. EL-4 was added to cultures of bone marrow cells in which colony formation was stimulated with several colony-stimulating factors (CSFs): monocyte CSF (M-CSF), granulocyte CSF (G-CSF), IL-3, IL-5 and conditioned medium from phytohemagglutinin-stimulated peripheral blood cells (PHA-CM). Inhibition of monocytic colony formation by DL-4 was observed in cultures that were stimulated with EL-3 or with PHA-CM, similar to the inhibition in M-CSF- or GM-CSF-stimulated cultures. The enhancement of granulocytic colony formation by IL-4 was restricted to G-CSF-induced colony growth. No enhancement was observed in cultures that were stimulated with IL-5 or with PHA-CM from which the G-CSF was neutralized by anti-G-CSF antibodies. Both the inhibiting and enhancing effects of IL-4 were preserved in cultures that were stimulated with concentrations of CSF that exceeded more than tenfold the plateau concentrations. Enhancement of granulocytic and inhibition of monocytic colony formation by IL-4 occurred simultaneously in cultures stimulated with PHA-CM or with a combination of G-CSF and M-CSF. In summary, we show that the inhibiting effect of IL- 4 on monocytic colony formation is independent of the growth factor used, whereas the stimulatory effect of IL-4 on granulocytic colony formation is restricted to G-CSF-induced cultures. Simultaneous occurrence of both effects results in preferential growth of granulocytes. |
| Databáze: | OpenAIRE |
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