Antimalarials and Phytotoxins from Botryosphaeria dothidea Identified from a Seed of Diseased Torreya taxifolia
| Autor: | M Kumarihamy, Babu L. Tekwani, Stephen O. Duke, N. P. Dhammika Nanayakkara, Shabana I. Khan, Daneel Ferreira, Edward M. Croom, Luiz H. Rosa, Natascha Techen |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
malaria Pharmaceutical Science Botryosphaeria dothidea Biology 01 natural sciences Endophyte Analytical Chemistry Drug Discovery parasitic diseases Physical and Theoretical Chemistry Plastid Apicoplast phytotoxin 010405 organic chemistry Torreya taxifolia Organic Chemistry fungi food and beverages Phytotoxin biology.organism_classification 0104 chemical sciences Metabolic pathway γ-lactam alkaloids Biochemistry Chemistry (miscellaneous) plant pathogenic fungi Molecular Medicine Phytotoxicity 010606 plant biology & botany |
| Zdroj: | Molecules Volume 26 Issue 1 |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules26010059 |
| Popis: | The metabolic pathways in the apicoplast organelle of Plasmodium parasites are similar to those in plastids in plant cells and are suitable targets for malaria drug discovery. Some phytotoxins released by plant pathogenic fungi have been known to target metabolic pathways of the plastid thus, they may also serve as potential antimalarial drug leads. An EtOAc extract of the broth of the endophyte Botryosphaeria dothidea isolated from a seed collected from a Torreya taxifolia plant with disease symptoms, showed in vitro antimalarial and phytotoxic activities. Bioactivity-guided fractionation of the extract afforded a mixture of two known isomeric phytotoxins, FRT-A and flavipucine (or their enantiomers, sapinopyridione and (-)-flavipucine), and two new unstable &gamma lactam alkaloids dothilactaenes A and B. The isomeric mixture of phytotoxins displayed strong phytotoxicity against both a dicot and a monocot and moderate cytotoxicity against a panel of cell lines. Dothilactaene A showed no activity. Dothilactaene B was isolated from the active fraction, which showed moderate in vitro antiplasmodial activity with high selectivity index. In spite of this activity, its instability and various other biological activities shown by related compounds would preclude it from being a viable antimalarial lead. |
| Databáze: | OpenAIRE |
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