Liquid chromatography/tandem mass spectrometry for the determination of fluoxetine and its main active metabolite norfluoxetine in human plasma with deuterated fluoxetine as internal standard
| Autor: | Qingxiang Shao, Zhouhua Ji, Meiyun Zhang, Cheng Yu, Jieyu Dai, Ping Liu, Fang Xu, Jiang Zhen, Hao Yuan, Bin Huang, Chuan Li, Fajun Nan, Jian Cui |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Quality Control Calibration curve Metabolite Tandem mass spectrometry Mass Spectrometry Analytical Chemistry Fluoxetine Hydrochloride chemistry.chemical_compound Liquid chromatography–mass spectrometry Fluoxetine Humans Spectroscopy Active metabolite Detection limit Chromatography Organic Chemistry Selected reaction monitoring Reproducibility of Results Reference Standards Deuterium chemistry Area Under Curve Calibration Solvents Antidepressive Agents Second-Generation Chromatography Liquid Half-Life |
| Zdroj: | Rapid communications in mass spectrometry : RCM. 16(19) |
| ISSN: | 0951-4198 |
| Popis: | Fluoxetine (F) and its N-demehylated metabolite norfluoxetine (NF) are selective inhibitors of serotonin reuptake in humans. A new sensitive rapid method for the simultaneous determination of F and NF in plasma was established and validated, and was further applied to assess the bioequivalence of two oral formulations of F in 22 healthy Chinese male volunteers who received a single oral dose of each formulation (containing 20 mg of fluoxetine hydrochloride). The new method involves using liquid chromatography/tandem mass spectrometry (LC/MS/MS) in multiple reaction monitoring mode with deuterated fluoxetine (DF) as internal standard. High levels of analytical sensitivity and specificity of MS/MS detection enabled use of a simple liquid-liquid extraction procedure. The combination of a simple sample clean-up procedure and short chromatographic run-time (5 min) considerably increased the productivity of the analytical method. The method was validated for the plasma concentration range 0.27–22 ng/mL for both of the test compounds, and the calibration curves were linear with coefficients of correlation >0.999. The limit of detection was 0.1 ng/mL for plasma F and NF. Taking the plasma sample size (200 µL) into account the new method for determination of F and NF is more sensitive than those described previously. Copyright © 2002 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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