Loss of PAR-3 protein expression is associated with invasion, lymph node metastasis, and poor survival in esophageal squamous cell carcinoma
Autor: | Osamu Dohi, Kohichiroh Yasui, Tomoko Kitaichi, Kei Terasaki, Kanji Yamaguchi, Yoshio Sumida, Nobuhisa Yamada, Naoto Iwai, Yoshito Itoh, Hironori Mitsuyoshi, Atsushi Umemura, Yuji Naito, Taichiro Nishikawa, Yasuyuki Gen, Yoh Zen, Michihisa Moriguchi, Akira Tomie |
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Rok vydání: | 2017 |
Předmět: |
Adult
Male 0301 basic medicine Pathology medicine.medical_specialty Time Factors Esophageal Neoplasms Biopsy Down-Regulation Cell Cycle Proteins Kaplan-Meier Estimate Biology Pathology and Forensic Medicine Metastasis 03 medical and health sciences Basal (phylogenetics) Claudin-1 Cell polarity Biomarkers Tumor medicine Humans Neoplasm Invasiveness Neoplasms Squamous Cell Esophagus Adaptor Proteins Signal Transducing Aged Neoplasm Staging Proportional Hazards Models Retrospective Studies Aged 80 and over Tight junction Membrane Proteins Middle Aged medicine.disease Immunohistochemistry digestive system diseases Epithelium 030104 developmental biology medicine.anatomical_structure Cytoplasm Cell culture Lymphatic Metastasis Carcinoma Squamous Cell Zonula Occludens-1 Protein Female Esophageal Squamous Cell Carcinoma |
Zdroj: | Human Pathology. 62:134-140 |
ISSN: | 0046-8177 |
Popis: | Disrupted cell polarity is a feature of epithelial cancers. The partitioning defective 3 (PAR-3) protein, a key component of the PAR complex that regulates the polarization of cells, is involved in tight junction formation at epithelial cell-cell contacts. Our previous study detected a homozygous deletion of the PAR-3 gene in esophageal squamous cell carcinoma (ESCC) cell lines and frequent copy number loss of the PAR-3 gene in primary ESCC. Here, we aimed to investigate the clinicopathological relevance of altered expression of the PAR-3 protein in primary ESCC. We immunohistochemically analyzed expression of the PAR-3 protein, as well as that of other tight junction proteins, ZO-1 and claudin-1, in 74 primary ESCCs. While the PAR-3 protein was expressed in the cytoplasm of basal cells, it was localized on the plasma membrane of suprabasal cells of normal squamous epithelium of the esophagus. Of the 74 ESCC tumors, 20 (27%), 11 (15%), and 13 (18%) were negative for PAR-3, ZO-1, and claudin-1 proteins, respectively. Negative PAR-3 protein expression, but not negative ZO-1 or claudin-1 expression, was significantly associated with deeper tumor invasion (P |
Databáze: | OpenAIRE |
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