Loss of PAR-3 protein expression is associated with invasion, lymph node metastasis, and poor survival in esophageal squamous cell carcinoma

Autor: Osamu Dohi, Kohichiroh Yasui, Tomoko Kitaichi, Kei Terasaki, Kanji Yamaguchi, Yoshio Sumida, Nobuhisa Yamada, Naoto Iwai, Yoshito Itoh, Hironori Mitsuyoshi, Atsushi Umemura, Yuji Naito, Taichiro Nishikawa, Yasuyuki Gen, Yoh Zen, Michihisa Moriguchi, Akira Tomie
Rok vydání: 2017
Předmět:
Adult
Male
0301 basic medicine
Pathology
medicine.medical_specialty
Time Factors
Esophageal Neoplasms
Biopsy
Down-Regulation
Cell Cycle Proteins
Kaplan-Meier Estimate
Biology
Pathology and Forensic Medicine
Metastasis
03 medical and health sciences
Basal (phylogenetics)
Claudin-1
Cell polarity
Biomarkers
Tumor
medicine
Humans
Neoplasm Invasiveness
Neoplasms
Squamous Cell
Esophagus
Adaptor Proteins
Signal Transducing
Aged
Neoplasm Staging
Proportional Hazards Models
Retrospective Studies
Aged
80 and over
Tight junction
Membrane Proteins
Middle Aged
medicine.disease
Immunohistochemistry
digestive system diseases
Epithelium
030104 developmental biology
medicine.anatomical_structure
Cytoplasm
Cell culture
Lymphatic Metastasis
Carcinoma
Squamous Cell
Zonula Occludens-1 Protein
Female
Esophageal Squamous Cell Carcinoma
Zdroj: Human Pathology. 62:134-140
ISSN: 0046-8177
Popis: Disrupted cell polarity is a feature of epithelial cancers. The partitioning defective 3 (PAR-3) protein, a key component of the PAR complex that regulates the polarization of cells, is involved in tight junction formation at epithelial cell-cell contacts. Our previous study detected a homozygous deletion of the PAR-3 gene in esophageal squamous cell carcinoma (ESCC) cell lines and frequent copy number loss of the PAR-3 gene in primary ESCC. Here, we aimed to investigate the clinicopathological relevance of altered expression of the PAR-3 protein in primary ESCC. We immunohistochemically analyzed expression of the PAR-3 protein, as well as that of other tight junction proteins, ZO-1 and claudin-1, in 74 primary ESCCs. While the PAR-3 protein was expressed in the cytoplasm of basal cells, it was localized on the plasma membrane of suprabasal cells of normal squamous epithelium of the esophagus. Of the 74 ESCC tumors, 20 (27%), 11 (15%), and 13 (18%) were negative for PAR-3, ZO-1, and claudin-1 proteins, respectively. Negative PAR-3 protein expression, but not negative ZO-1 or claudin-1 expression, was significantly associated with deeper tumor invasion (P
Databáze: OpenAIRE
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