Genotoxicity assessment of ammonia in cultured Oreochromis niloticus using RAPD assay
| Autor: | Shenouda M. Girgis, Iman M.K. Abumourad, Magdy I. Hanna |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
High concentration
Screening assay RAPD assay Biology medicine.disease_cause biology.organism_classification Molecular biology Treatment period Ammonia nitrogen RAPD Ammonia chemistry.chemical_compound Oreochromis chemistry medicine General Materials Science Food science O. niloticus Genotoxicity TAN |
| Zdroj: | Journal of Genetic Engineering and Biotechnology. (2):209-212 |
| ISSN: | 1687-157X |
| DOI: | 10.1016/j.jgeb.2012.10.002 |
| Popis: | Limited efforts have been made to study the genotoxic effects of ammonia in cultured OOreochromi niloticus . Therefore, the present study was planned to assess the genotoxic effect of ammonia in cultured O. niloticus using random amplified polymorphic DNA (RAPD) assay. Fish was categorized into four groups. The 1st group exposed to 2.5 mg/L of total ammonia nitrogen (TAN) (0.16 NH 3 mg/L), the 2nd exposed to 5.0 mg/L of total ammonia nitrogen (TAN) (0.32 NH 3 mg/L) and the 3rd exposed to 10.0 mg/L of total ammonia nitrogen (TAN) (0.65 NH 3 mg/L), in addition to control group for the treatment period of 6 days. The results revealed that some genes in O. niloticus are susceptible to DNA disturbances/mutation as a result of exposure to high concentration of ammonia in water, this clearly indicated using RAPD screening assay. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|