Isotopic analysis of Cu in blood serum by multi-collector ICP-mass spectrometry: a new approach for the diagnosis and prognosis of liver cirrhosis?
| Autor: | Hans Van Vlierberghe, Joris R. Delanghe, Sara Lauwens, Marta Costas-Rodríguez, Yulia Anoshkina, Frank Vanhaecke |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Liver Cirrhosis medicine.medical_specialty Cirrhosis Population Biophysics Biochemistry Gastroenterology Biomaterials Liver disease Blood serum Primary biliary cirrhosis Isotopes Internal medicine Ascites medicine Humans education Aged Aged 80 and over Principal Component Analysis education.field_of_study biology Chemistry Spectrophotometry Atomic Metals and Alloys Albumin Reproducibility of Results Middle Aged Reference Standards Prognosis medicine.disease Chemistry (miscellaneous) Isotope Labeling Immunology biology.protein Female medicine.symptom Ceruloplasmin Copper |
| Zdroj: | Metallomics. 7:491-498 |
| ISSN: | 1756-591X 1756-5901 |
| Popis: | The isotopic composition of blood serum Cu has been investigated as a potential parameter for the diagnosis and prognosis of liver cirrhosis. Serum samples from supposedly healthy women (reference population) and from a group of female patients suffering from liver cirrhosis of different etiologies were analysed. The procedure for isolation of serum Cu and the measurement protocol for its isotopic analysis by multi-collector inductively coupled plasma-mass spectrometry (MC-ICP-MS) were evaluated. Significant differences in the isotopic composition of Cu were observed between the reference population and the patients. A wide spread in δ(65)Cu was observed within the cirrhosis population and δ(65)Cu seems to be linked to the severity of the disease. Patients with end-stage liver disease showed a significantly lighter serum Cu isotopic composition. Many clinical parameters used for the diagnosis and monitoring of liver diseases, i.e. the levels of aspartate aminotransferase, De Ritis ratio, prothrombin and international normalized ratio, albumin, bilirubin, Na and C-reactive protein, correlate well with the δ(65)Cu values, as did the ceruloplasmin level and the ceruloplasmin/Cu concentration ratio. The isotopic composition of serum Cu appears to reveal the synthetic and hepatocellular function of the liver synergistically with inflammation and fluid retention in the cohort studied. A relevant relationship was also observed between δ(65)Cu and scores of mortality risk, such as the Model for End-stage Liver Disease (MELD) and MELD-Na. Thus, the isotopic composition of serum Cu shows potential as a new approach for the prognosis of liver disease, and although further investigation is required, for evaluation of the mortality risk in end-stage liver disease and prioritization of liver transplants. |
| Databáze: | OpenAIRE |
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