Additional file 2 of Two distinct conformers of PrPD type 1 of sporadic Creutzfeldt–Jakob disease with codon 129VV genotype faithfully propagate in vivo

Autor: Cali, Ignazio, Espinosa, Juan Carlos, Nemani, Satish K., Marin-Moreno, Alba, Camacho, Manuel V., Rabail Aslam, Tetsuyuki Kitamoto, Appleby, Brian S., Torres, Juan Maria, Gambetti, Pierluigi
Rok vydání: 2021
DOI: 10.6084/m9.figshare.14314055.v1
Popis: Additional file 2. Fig. S2: Characterization of mouse brain resPrPD following 2nd passage in Tg129M mice. T1 and its superscript atop the blot refer to the mouse resPrPD T1 variant; VV120 and VV121 refer to resPrPD harvested from sCJDVV1 controls. Mouse resPrPD showing a ~21–20 kDa doublet following 2nd passage with VV120 (lanes 2 & 3) and VV121–20* (lanes 5 & 6); lane 6: longer exposure time of resPrPD visualized in lane 5. No resPrPD was detected after serial passage with VV121 (lane 7); Neg.: negative. Licor near-infrared (lanes 1-6); chemiluminescence (lanes 7 & 8).
Databáze: OpenAIRE