Aptamer-based sensor for quantitative detection of mercury (II) ions by attenuated total reflection surface enhanced infrared absorption spectroscopy
| Autor: | Hsien-Chang Chang, Chia Wei Liu, Ruey-Jen Yang, Tien Chun Tsai, Masatoshi Osawa |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Spectrophotometry
Infrared Surface Properties Aptamer Analytical chemistry Infrared spectroscopy Biosensing Techniques 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry Ion Absorbance Environmental Chemistry Molecule Surface layer Spectroscopy Ions Chemistry 010401 analytical chemistry Electrochemical Techniques Mercury Aptamers Nucleotide 021001 nanoscience & nanotechnology 0104 chemical sciences Attenuated total reflection 0210 nano-technology |
| Zdroj: | Analytica Chimica Acta. 1033:137-147 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2018.05.037 |
| Popis: | A sensing platform based on the attenuated total reflection surface enhanced infrared absorption spectroscopy (ATR-SEIRAS) technique and immobilized aptamer has been proposed herein for the selective detection of mercury ions (Hg2+). In the proposed platform, 5′ thiolated 32-mer DNA probes with methylene blue at the 3′ end were immobilized on a thin gold (Au) surface layer. Following Hg2+ ions interacting with T bases of the aptamer, T-Hg-T bonds are formed; resulting in a hairpin-shaped formation of the DNA and a detectable change in the IR absorbance of the sensing interface. Notably, the background noise produced by external molecules (e.g., water, non-specific binding molecules and bulk solution) is reduced to a negligible level by means of the ATR detection mode. It is shown that the proposed sensor has a linear response (R2 = 0.986) with high sensitivity and good selectivity over the Hg2+ range of 0.01 μ M–50 μ M. |
| Databáze: | OpenAIRE |
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