Characterization of lncRNA LINC00520 and functional polymorphisms associated with breast cancer susceptibility in Chinese Han population

Autor: Mengmeng Song, Yanli Wang, Chunhua Song, Yan Ma, Qiaoyun Guo, Rui Peng, Linping Xu, Feifei Chong, Liping Dai
Rok vydání: 2019
Předmět:
0301 basic medicine
Adult
Cancer Research
China
Single-nucleotide polymorphism
Triple Negative Breast Neoplasms
Biology
Lower risk
lcsh:RC254-282
Polymorphism
Single Nucleotide
single‐nucleotide polymorphisms
susceptibility
03 medical and health sciences
0302 clinical medicine
Breast cancer
Chinese han population
breast cancer
lncRNA
Asian People
Gene Frequency
Genetic model
Genotype
medicine
Biomarkers
Tumor
Humans
Radiology
Nuclear Medicine and imaging
Genetic Predisposition to Disease
RNA
Messenger
Allele
Genotyping
Alleles
Original Research
Genetics
Molecular Epidemiology
Middle Aged
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
medicine.disease
Gene Expression Regulation
Neoplastic
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
LINC00520
Female
RNA
Long Noncoding
Cancer Prevention
Zdroj: Cancer Medicine
Cancer Medicine, Vol 9, Iss 6, Pp 2252-2268 (2020)
ISSN: 2045-7634
Popis: Background The aim was to evaluate the association between the LINC00520 genetic polymorphisms and breast cancer (BC) susceptibility. Methods Nine single‐nucleotide polymorphisms (SNPs) on LINC00520 genotyping were performed in 504 BC patients and 505 cancer‐free controls in Chinese Han population to study the relationship between LINC00520 polymorphism and BC susceptibility. qRT‐PCR and luciferase tests were used to explore how rs12880540 affected the expression of LINC00520. Results The genotype GG (OR:3.58, 95%CI:1.32‐9.69) in rs8012083 increased the risk of triple‐negative BC. The genotype GG (OR:0.31, 95%CI:0.14‐0.69) in rs8012083, the genotype AA (OR:2.74, 95%CI:1.01‐7.42) in rs2152275, and genotype TG (OR:1.62, 95%CI:1.04‐2.52) in rs12880540 were associated with HER‐2 status. The dominant (OR:0.65, 95%CI:0.45‐0.95) and overdominant genetic model (OR:0.67, 95%CI:0.46‐0.98) consistently showed that rs11622641 T was significantly associated with lower risk of BC. Similarly, the recessive genetic model (OR:1.57, 95%CI:1.07‐2.30) of rs12880540 and the dominant (OR:1.62, 95%CI:1.24‐2.11) and overdominant (OR:1.56, 95%CI:1.19‐2.03) genetic model of rs2152278 may increase the risk of BC. The relative expression of LINC00520 increased linearly with the increase in the number of rs12880540 mutations. rs12880540 alleles were due to the interaction between LINC00520 and miR‐3122 at T, but the mutation of rs12880540 G > T had no effect on the binding ability of LINC00520 and miR‐3122. Conclusion A genetic variant of rs8012083 in LINC00520 may be used as a biomarker for triple‐negative BC after further evaluation of diagnostic tests. The genetic variant of LINC00520 was related to the susceptibility of BC, and rs12880540 might affect the corresponding mRNA expression of lncRNA LINC00520.
In our epidemiology study, nine single‐nucleotide polymorphisms (SNPs) in lncRNA LINC00520 were selected for genotyping among 504 breast cancer cases and 505 healthy controls. The 504 cases are all new breast cancer patients which were pathologically confirmed and the controls were from an epidemiological survey of cardiovascular disease in Henan Province with 20 000 participants and they were included without a history of cancer or other diseases. A genetic variant of rs8012083 in LINC00520 could be used as a biomarker for triple‐negative BC. The genetic variants of lncRNA LINC00520 were associated with the susceptibility of BC, and rs12880540 might affect the corresponding mRNA expression of lncRNA LINC00520.
Databáze: OpenAIRE
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