Chemical characterization and biotechnological applicability of pigments isolated from Antarctic bacteria
| Autor: | Lorena Rigo Gaspar Cordeiro, Luiz H. Rosa, Iramaia Angélica Neri-Numa, Hosana Maria Debonsi, Ramon Canela-Garayoa, Renata Spagolla Napoleão Tavares, Jordi Eras, Marili V.N. Rodrigues, Valéria Maia de Oliveira, Tiago Rodrigues e Silva, José A. A. Schultz, Glaucia Maria Pastore |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antarctic Regions 01 natural sciences Applied Microbiology and Biotechnology Industrial Microbiology 03 medical and health sciences chemistry.chemical_compound Pigment Phytoene 010608 biotechnology Arthrobacter Food science Carotenoid chemistry.chemical_classification biology MICROBIOLOGIA INDUSTRIAL Arthrobacter agilis Arthrobacter psychrochitiniphilus Pigments Biological biology.organism_classification Carotenoids Zeaxanthin 030104 developmental biology chemistry visual_art visual_art.visual_art_medium Flavobacteriaceae Bacteria Biotechnology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Considering the global trend in the search for alternative natural compounds with antioxidant and sun protection factor (SPF) boosting properties, bacterial carotenoids represent an opportunity for exploring pigments of natural origin which possess high antioxidant activity, lower toxicity, no residues, and no environmental risk and are readily decomposable. In this work, three pigmented bacteria from the Antarctic continent, named Arthrobacter agilis 50cyt, Zobellia laminarie 465, and Arthrobacter psychrochitiniphilus 366, were able to withstand UV-B and UV-C radiation. The pigments were extracted and tested for UV absorption, antioxidant capacity, photostability, and phototoxicity profile in murine fibroblasts (3T3 NRU PT–OECD TG 432) to evaluate their further potential use as UV filters. Furthermore, the pigments were identified by ultra-high-performance liquid chromatography–photodiode array detector–mass spectrometry (UPLC-PDA-MS/MS). The results showed that all pigments presented a very high antioxidant activity and good stability under exposure to UV light. However, except for a fraction of the A. agilis 50cyt pigment, they were shown to be phototoxic. A total of 18 different carotenoids were identified from 23 that were separated on a C18 column. The C50 carotenes bacterioruberin and decaprenoxanthin (including its variations) were confirmed for A. agilis 50cyt and A. psychrochitiniphilus 366, respectively. All-trans-bacterioruberin was identified as the pigment that did not express phototoxic activity in the 3T3 NRU PT assay (MPE |
| Databáze: | OpenAIRE |
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