Miniaturized pH Holographic Sensors for the Monitoring of Lactobacillus casei Shirota Growth in a Microfluidic Chip
| Autor: | Leon Chan, Gita Khalili Moghaddam, Zhiping Wang, Christopher R. Lowe |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Lactobacillus casei
Materials science Holography Bioengineering Nanotechnology 02 engineering and technology 01 natural sciences pH meter law.invention Acetone law Lab-On-A-Chip Devices Bioreactor Dimethylpolysiloxanes Bioprocess Instrumentation Fluid Flow and Transfer Processes Holographic sensor biology Process Chemistry and Technology 010401 analytical chemistry Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Lacticaseibacillus casei Nylons Microfluidic chip Methacrylates 0210 nano-technology Biosensor |
| Zdroj: | ACS sensors. 4(2) |
| ISSN: | 2379-3694 |
| Popis: | Bioreactors have been used both to develop new, and to improve bioprocess yields for, biopharmaceutical products. However, efforts to miniaturize bioreactors, in order to save costs and accelerate process development times, have been limited by the lack of on-site monitoring capabilities available at such scales. In this study, small volume (3 nL) nonconsumptive holographic sensors were integrated into a glass-PDMS microfluidic chip to monitor via a blue-shift in the resultant holographic replay wavelength, the change in pH during microbial growth of Lactobacillus casei ( L. casei) Shirota. Within the optimal growth pH range of L. casei, the accuracy of the miniaturized pH sensors was comparable to that of a conventional pH meter. Conceivably, this approach could be extrapolated to an array of miniaturized holographic sensors sensitive to different analytes, and thereby paving the way for reliable, real-time, noninvasive monitoring of microorganisms in a nanobioreactor. |
| Databáze: | OpenAIRE |
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