Characterization of the Plant Growth-Promoting Activities of Endophytic Fungi Isolated from Sophora flavescens
| Autor: | Biljana Škrbić, Enkh-Amgalan Jigjiddorj, László Bakacsy, Adiyadolgor Turbat, Csaba Vágvölgyi, Ágnes Szepesi, Aruna Vigneshwari, Huynh Thu, Dávid Rakk, Sándor Kocsubé, András Szekeres |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Microbiology (medical) Fusarium Siderophore Biology 01 natural sciences Microbiology Plant use of endophytic fungi in defense Article 03 medical and health sciences Virology Botany Bioassay Arabidopsis thaliana plant growth-promoting activities Carotenoid lcsh:QH301-705.5 chemistry.chemical_classification endophytic fungi Sophora flavescens fungi food and beverages biology.organism_classification Alternaria 030104 developmental biology IAA (indole-acetic-acid) production chemistry lcsh:Biology (General) 010606 plant biology & botany |
| Zdroj: | Microorganisms, Vol 8, Iss 683, p 683 (2020) Microorganisms Volume 8 Issue 5 |
| ISSN: | 2076-2607 |
| Popis: | Endophytic fungi in symbiotic association with their host plant are well known to improve plant growth and reduce the adverse effects of both biotic and abiotic stresses. Therefore, fungal endophytes are beginning to receive increased attention in an effort to find growth-promoting strains that could be applied to enhance crop yield and quality. In our study, the plant growth-promoting activities of endophytic fungi isolated from various parts of Sophora flavescens (a medicinally important plant in Mongolia and China) have been revealed and investigated. Fungal isolates were identified using molecular taxonomical methods, while their plant growth-promoting abilities were evaluated in plate assays. Altogether, 15 strains were isolated, representing the genera Alternaria, Didymella, Fusarium and Xylogone. Five of the isolates possessed phosphate solubilization activities and twelve secreted siderophores, while all of them were able to produce indoleacetic acid (IAA) in the presence or absence of tryptophan. The endogenous and exogenous accumulation of IAA were also monitored in liquid cultures using the HPLC-MS/MS technique to refine the plate assay results. Furthermore, for the highest IAA producer fungi, the effects of their extracts were also examined in plant bioassays. In these tests, the primary root lengths of the model Arabidopsis thaliana were increased in several cases, while the biomasses were significantly lower than the control IAA treatment. Significant alterations have also been detected in the photosynthetic pigment (chlorophyll-a, -b and carotenoids) content due to the fungal extract treatments, but these changes did not show any specific trends. |
| Databáze: | OpenAIRE |
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