Cigarette smoking decreases bioactive interleukin-6 secretion by alveolar macrophages
| Autor: | Diaa M. Soliman, Homer L. Twigg |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Pulmonary and Respiratory Medicine
Adult Lipopolysaccharides Male medicine.medical_specialty Physiology medicine.medical_treatment Stimulation Enzyme-Linked Immunosorbent Assay Immune system Physiology (medical) Internal medicine medicine Humans Secretion Antigens Interleukin 6 biology business.industry Interleukin-6 Macrophages Smoking Interleukin Cell Biology Pulmonary Alveoli Cytokine Endocrinology Alveolar macrophage biology.protein Female business Intracellular Interleukin-1 |
| Zdroj: | The American journal of physiology. 263(4 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | Studies suggest smokers have decreased alveolar macrophage (AM) accessory cell (AC) function and a reduced incidence of immune-mediated lung diseases such as sarcoidosis. Impaired AM secretion of cytokines important in T-cell immune responses could explain this observation. We investigated production and secretion of interleukin-1 (IL-1) and interleukin-6 (IL-6) in smokers and nonsmokers. Lipopolysaccharide-induced AM IL-1 secretion in smokers was significantly reduced compared with nonsmoker AM. However, intracellular IL-1 in smoker AM was higher than in nonsmokers, suggesting that reduced IL-1 secretion was due to impaired release rather than reduced production. Smoker AM secreted significantly less bioactive IL-6 measured in a bioassay compared with nonsmoker AM. Intracellular IL-6 was virtually undetectable in both groups. In some smokers IL-6 production determined by immunoprecipitation was reduced. However, as a group antigenic IL-6 secretion determined by enzyme-linked immunoabsorbent assay was similar in smokers and nonsmokers, suggesting that smoker AM may cosecrete an inhibitor of IL-6 bioactivity. Indeed, AM supernatants from smokers inhibited B9 proliferation in response to maximal recombinant IL-6 stimulation, whereas supernatants from nonsmokers did not. We conclude that AM from smokers secrete less cytokines important in T-cell proliferation than AM from nonsmokers and suggest that for IL-6 this impairment is related to both decreased production of antigenic protein as well as cosecretion of an IL-6 inhibitor. |
| Databáze: | OpenAIRE |
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