Identification of differentially abundant mRNA transcripts and autocrine/paracrine factors in oocytes and follicle cells of mud crabs
| Autor: | Yanan Yang, Chenchang Bao, Yan Zhuang, Zhaoxia Cui, Chenye Zhang, Yi Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Brachyura
Cyclin A Cyclin B Biology 03 medical and health sciences Paracrine signalling 0302 clinical medicine Endocrinology Food Animals Ovarian Follicle medicine Animals RNA Messenger Autocrine signalling Cyclin 030219 obstetrics & reproductive medicine Germinal vesicle 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine Oocyte 040201 dairy & animal science Hormones Cell biology medicine.anatomical_structure Gene Expression Regulation biology.protein Oocytes Animal Science and Zoology Female Vitellogenesis |
| Zdroj: | Animal reproduction science. 230 |
| ISSN: | 1873-2232 |
| Popis: | The late vitellogenic stage of the mud crab is characterized by large and obvious follicle cells as well as an enlarged oocyte nucleus and a prominent germinal vesicle (GV). The aim of this study was evaluation of functions of oocytes and follicle cells during meiosis as well as at identifying associated ovarian autocrine/paracrine factors using comparative transcriptomics. The results from the KEGG pathway analysis indicated DNA replication, nucleotide excision repair, spliceosome and the ribosome pathways were highly associated with oocyte maturation across both transcriptomes. In addition, there was a larger abundance of mRNA transcripts for cell cycle-related genes in the oocyte, as well as cyclin A, cyclin B and CKS1B in the GV than at the time of germinal vesicle breakdown (GVBD). These findings indicate these cell cycle-related genes might be involved in GVBD induction. Results when there was localization of ligands and the respective receptors of VEGF, TGFβ propeptide and BMP9/10 indicated these proteins might be autocrine/paracrine factors. Results from functional analysis of VEGF, TGFβ propeptide and BMP9/10 in oocyte maturation using RNA interference revealed that these proteins might be involved in oocyte maturation by regulating cyclin abundance. This is the first study on the functions of VEGF in oocyte maturation in invertebrates. |
| Databáze: | OpenAIRE |
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