Protein tyrosine phosphatase, receptor type B is a potential biomarker and facilitates cervical cancer metastasis via epithelial-mesenchymal transition
| Autor: | Min Zhang, Xiao-Cong Jiang, Peng-Juan Liao, Xiu-Ping Wang, Ying-Xia Liu, Zhuo-Ya Huang, Ming Zhong, Ai-Hua Sun |
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| Rok vydání: | 2021 |
| Předmět: |
Epithelial-Mesenchymal Transition
cervical cancer Uterine Cervical Neoplasms Bioengineering Protein tyrosine phosphatase Biology Applied Microbiology and Biotechnology Metastasis PTPRB Western blot Databases Genetic tcga database Biomarkers Tumor medicine Humans metastasis Epithelial–mesenchymal transition Neoplasm Metastasis KEGG medicine.diagnostic_test Cell growth Receptor-Like Protein Tyrosine Phosphatases Class 3 General Medicine medicine.disease Cancer research biomarker Immunohistochemistry Female ptprb TP248.13-248.65 Research Article Research Paper Biotechnology |
| Zdroj: | Bioengineered, Vol 12, Iss 1, Pp 5739-5748 (2021) Bioengineered article-version (VoR) Version of Record |
| ISSN: | 2165-5987 2165-5979 |
| DOI: | 10.1080/21655979.2021.1968250 |
| Popis: | Cervical cancer (CC) is one of the most common malignant tumors. This study analyzed the impact of protein tyrosine phosphatase, receptor type B (PTPRB) on malignant behavior of CC and explored its possible molecular mechanism. RT-PCR, western blot and Immunohistochemistry were applied to examine the expression of PTPRB in CC specimens and cells. Aberrant PTPRB expression in CC and survival outcomes were constructed using The Cancer Genome Atlas (TCGA) database and tissue microarray cervical squamous cell carcinoma cohort. Cultured human CC cells were assayed for viability, apoptosis, migration, and invasion in vitro and in vivo. Kyoto Encyclopedia of Genes and Genomes (KEGG) assays and gene set enrichment analysis (GSEA) assays were used to delve into PTPRB-related pathways using TCGA datasets. The levels of proteins associated with the epithelial–mesenchymal transition (EMT) pathway and modulated by PTPRB were examined through Western blot. We found that the levels of PTPRB in CC tissues and cells were distinctly up-regulated. PTPRB was also an unfavorable prognostic factor for CC patients. Functionally, PTPRB knockdown exhibits tumor-suppressive function via reducing cell proliferation and metastasis and inducing cell apoptosis. KEGG assays and GSEA assays suggested PTPRB overexpression was associated with several tumor-related pathways. The results of Western blot assays suggested that N-cadherin was decreased in the PTPRB-knockdown CC cells, while E-cadherin was increased. Overall, PTPRB is highly expressed in CC and can effectively enhance the proliferation, metastasis and EMT process of tumor cells. PTPRB is expected to be a therapeutic target for CC. |
| Databáze: | OpenAIRE |
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