Embryo cell allocation patterns are not altered by biopsy but can be linked with further development
| Autor: | Nadia Islam, Lessly P. Sepulveda-Rincon, Peter Marsters, Walid E. Maalouf, Nathalie Beaujean, Bruce K. Campbell |
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| Přispěvatelé: | Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Institut cellule souche et cerveau / Stem Cell and Brain Research Institute (U1208 Inserm - UCBL1 / SBRI - USC 1361 INRAE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut cellule souche et cerveau / Stem Cell and Brain Research Institute (SBRI), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Litter (animal) Blastomeres Embryology Biopsy Organogenesis [SDV]Life Sciences [q-bio] Cell Count Embryo Culture Techniques Mice Endocrinology Pregnancy Birth Weight Inner cell mass [SDV.BDD]Life Sciences [q-bio]/Development Biology ComputingMilieux_MISCELLANEOUS medicine.diagnostic_test [SDV.BA]Life Sciences [q-bio]/Animal biology Obstetrics and Gynecology Embryo Embryo transfer medicine.anatomical_structure embryonic structures Female medicine.medical_specialty Offspring Cleavage Stage Ovum Biology Andrology 03 medical and health sciences Species Specificity Embryo biopsy pre-patterning PGD/PGS organ development embryo transfer implantation Internal medicine medicine Animals Embryo Implantation Blastocyst Body Patterning Research [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Cell Biology Blastomere Embryo Transfer 030104 developmental biology Animals Newborn Reproductive Medicine |
| Zdroj: | Reproduction Reproduction, BioScientifica, 2017, 154 (6), pp.807-814. ⟨10.1530/REP-17-0514⟩ Reproduction (Cambridge, England) |
| ISSN: | 1470-1626 1741-7899 |
| DOI: | 10.1530/REP-17-0514⟩ |
| Popis: | International audience; It has been suggested that first embryo cleavage can be related with the embryonic-abembryonic axis at blastocyst stage in mice. Thus, cells of the 2-cell embryo might be already biased to form the inner cell mass or trophectoderm. This study was conducted to observe the possible effects of embryo biopsy on cell allocation patterns during embryo preimplantation in two different mouse strains and the effects of these patterns on further development. First, one blastomere of the 2-cell embryo was injected with a lipophilic tracer and cell allocation patterns were observed at blastocyst stage. Blastocysts were classified into orthogonal, deviant or random pattern. For the first experiment, embryos were biopsied at 8-cell stage and total cell counts (TCC) were annotated. Furthermore, non-biopsied blastocysts were transferred into foster mothers. Then, pups and their organs were weighed two weeks after birth. Random pattern was significantly recurrent (≈60%), against orthogonal ( |
| Databáze: | OpenAIRE |
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